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関連する概念動画

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

7.7K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
7.7K
Proteomics01:33

Proteomics

9.9K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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SDS-PAGE01:27

SDS-PAGE

34.0K
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
34.0K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.7K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Updated: Feb 20, 2026

Proteomic Profiling of Macrophages by 2D Electrophoresis
07:53

Proteomic Profiling of Macrophages by 2D Electrophoresis

Published on: November 4, 2014

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2D-DIGE プロテオミクス

Kay Ohlendieck1, Paul Dowling1

  • 1Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland; Kathleen Lonsdale Institute for Human Health Research, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland.

Advances in clinical chemistry
|February 18, 2026
PubMed
まとめ
この要約は機械生成です。

二次元差異ゲル電泳 (2D-DIGE) は,比較プロテオミクスのための敏感なタンパク質分離を提供します. このレビューでは,生物サンプルにおけるバイオマーカーの発見のための2D-DIGEの進歩を調査します.

キーワード:
差異ゲル電泳は,差異ゲルの電泳である.光ラベル 画像分析 アイソエレクトリック フォーカシング プロテオミクス マススペクトロメトリー 質量スペクトロメトリー

さらに関連する動画

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting
10:51

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting

Published on: April 10, 2014

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Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
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Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

Published on: April 2, 2018

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関連する実験動画

Last Updated: Feb 20, 2026

Proteomic Profiling of Macrophages by 2D Electrophoresis
07:53

Proteomic Profiling of Macrophages by 2D Electrophoresis

Published on: November 4, 2014

13.4K
Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting
10:51

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting

Published on: April 10, 2014

16.8K
Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
12:34

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

Published on: April 2, 2018

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科学分野:

  • バイオケミストリー バイオケミストリー
  • プロテオミクス プロテオミクスは,プロテオミクスの
  • アナリティカル・ケミストリー (Analytical Chemistry) とは

背景:

  • ゲル電泳は,生化学におけるタンパク質分離に不可欠である.
  • 微分光タグ付けは,比較プロテオミクス研究における変動を最小限に抑えます.
  • この目的のために二次元差異ゲル電泳 (2D-DIGE) が広く使用されています.

研究 の 目的:

  • 2D-DIGEにおける最近の進歩をレビューする.
  • プロテオミクスの比較応用に焦点を当てること.
  • バイオマーカーの発見のための2D-DIGEの適性を調査するために.

主な方法:

  • 2D-DIGEに関する最近の文献のレビュー.
  • 敏感性を高めるための戦略の分析.
  • バイオアナリティクスの利点と限界についての議論.

主要な成果:

  • 2D-DIGEは,ゲルベースのプロテオミック分析の感度が向上しています.
  • このテクニックは,比較研究において,バイオアナリティクスの利点をもたらします.
  • 技術的な制限は,アプリケーションの文脈で議論されます.

結論:

  • 2D-DIGEは,セルラーシステムとバイオフリウイドの大規模スクリーニングのための貴重なツールです.
  • 複雑な生物サンプルにおけるバイオマーカーの発見をサポートします.
  • さらなる進歩により,比較プロテオミクスにおけるその有用性が強化されています.