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Updated: Feb 20, 2026

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
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In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

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エンジニアリングされたUn1Cas12f1は,強化された活動とターゲティング範囲のマルチプレックスゲノム編集のために設計されました

Yanan Huo1, Jiale Mei1, Dan Zhang1

  • 1Shanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai, China.

Nature communications
|February 18, 2026
PubMed
まとめ

エンジニアリングされたCRISPR-Cas12f (evoCas12f) は,PAM認識を拡張し,遺伝子編集の効率を高める. この最適化されたシステムは,マウスのホモジゴス変異を含む精密なゲノム工学を可能にし,治療上の応用を進めています.

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CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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