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Updated: Apr 30, 2026

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
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Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

Published on: February 15, 2012

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アコースティック強化フローサイトメトリーによるバクテリアのグラム分化

Xiao Xuan Huang1, Nadezda Urosevic1,2,3, Timothy J J Inglis1,2,3

  • 1Department of Microbiology, PathWest Laboratory Medicine, QEII Medical Centre, Nedlands, WA, Australia.

Journal of medical microbiology
|February 19, 2026
PubMed
まとめ

SYTO 9染料を用いた音響強化フローサイトメトリーは,グラム陽性菌とグラム陰性菌を効果的に区別します. この方法は,細菌のグラム染色に新しい単一染料のアプローチを提供し,臨床微生物学の診断を改善します.

キーワード:
バクテリアのグラム分化.グラムステインステインアコースティック・フロー・サイトメトリー (AFC)ブルーレーザーチャンネル3高度 (BL3-H)光染料 SYTO 9 光染料 SYTO 9 光染料について

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Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a...
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