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Gene Evolution - Fast or Slow?02:05

Gene Evolution - Fast or Slow?

The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Epistasis Analysis01:09

Epistasis Analysis

Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...
Gene Evolution - Fast or Slow?02:05

Gene Evolution - Fast or Slow?

The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
Evolutionary Processes in Microbes01:26

Evolutionary Processes in Microbes

Microbial evolution occurs rapidly due to short generation times and a variety of genetic processes, including horizontal gene transfer, mutation, recombination, and genetic drift. These mechanisms collectively enable microbes to adapt swiftly to changing environments.Horizontal gene transfer (HGT) allows genes to move between different species and occurs through three main mechanisms: conjugation, transformation, and transduction. Conjugation involves direct cell-to-cell contact for DNA...
Evolution of New Traits in Microbes01:24

Evolution of New Traits in Microbes

Microorganisms evolve rapidly due to their large population sizes and short generation times, often exhibiting measurable changes within days under laboratory conditions. Natural selection acts on standing genetic variation, enabling the retention and amplification of beneficial traits that confer fitness advantages in changing environments.Adaptive Pigment Regulation in RhodobacterIn Rhodobacter, a genus of purple non-sulfur bacteria, light-harvesting pigments such as bacteriochlorophyll and...

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関連する実験動画

Updated: Jun 20, 2026

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
09:01

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Published on: March 16, 2011

タンパク質の言語モデルとエピスタティック相互作用によって導かれる急速な導かれた進化.

Vincent Q Tran1,2, Matthew Nemeth1, Liam J Bartie1

  • 1Arc Institute, 3181 Porter Drive, Palo Alto, CA, USA.

Science (New York, N.Y.)
|February 19, 2026
PubMed
まとめ

私たちはMULTI-evolveを開発し,シネジスティック変異を効率的に発見するための急速なタンパク質エンジニアリングフレームワークを開発しました. この機械学習によるアプローチは,改善されたタンパク質機能の発見を加速します.

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Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

A Practical Guide to Phage- and Robotics-Assisted Near-Continuous Evolution
05:08

A Practical Guide to Phage- and Robotics-Assisted Near-Continuous Evolution

Published on: January 12, 2024

関連する実験動画

Last Updated: Jun 20, 2026

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
09:01

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Published on: March 16, 2011

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

A Practical Guide to Phage- and Robotics-Assisted Near-Continuous Evolution
05:08

A Practical Guide to Phage- and Robotics-Assisted Near-Continuous Evolution

Published on: January 12, 2024

科学分野:

  • バイオケミストリーと分子生物学
  • コンピュータ生物学 コンピュータ生物学
  • プロテイン工学は,タンパク質の

背景:

  • タンパク質工学は,シネギスティック変異のための広大な配列空間をナビゲートする上で課題に直面しています.
  • 段階的な変異スタッキングや機械学習のような現在の方法は,しばしば非効率的または資源密集的である.

研究 の 目的:

  • MULTI-evolveを導入し,タンパク質のマルチミュータントを体系的に設計するための新しい枠組みを導入する.
  • 遺伝子合成の限界を克服し,有益な突然変異の発見を加速する.

主な方法:

  • タンパク質言語モデルや機能データとエピスタティックモデリングを組み合わせて,シネギスティックな突然変異の組み合わせを予測する.
  • マルチアセンブリ (MULTI-assembly) を使用し,マルチキロベース配列をアセンブリするための高効率のミュータゲネシス技術である.

主要な成果:

  • 3つの異なるタンパク質を対象とした進化の1つのラウンドで,最大10倍のタンパク質の改善を達成しました.
  • エンドツーエンドのマルチミュータントエンジニアリングを合理化するフレームワークの能力を実証しました.

結論:

  • MULTI-evolveは,機能強化されたタンパク質を設計するための迅速かつ効率的なソリューションを提供します.
  • このフレームワークは,幅広い種類のタンパク質と機能に適用できます.