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IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

15.2K
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
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Golgi Matrix Proteins01:12

Golgi Matrix Proteins

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Golgi matrix proteins are a group of highly dynamic proteins that maintain the stacked structure of Golgi. These proteins adapt to rapid morphological changes of the Golgi during the cell cycle. During cell division, mild proteolysis removes these connections resulting in Golgi unstacking. In The daughter cells, these proteins help reassemble the unstacked Golgi.
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
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GPI Anchoring of Proteins in the ER Membrane01:29

GPI Anchoring of Proteins in the ER Membrane

5.6K
GPI-anchoring is a post-translational, reversible protein modification that is ubiquitous in eukaryotes. Such proteins are primarily present on the exoplasmic leaflet of the plasma membrane.
GPI-anchor structure
A sequence of 11 enzymatic reactions results in the synthesis of the complete GPI anchor consisting of a hydrophobic and a hydrophilic portion. The hydrophobic portion comprises phosphatidylinositol, while the hydrophilic part comprises polar groups like phosphoethanolamine,...
5.6K
Intracellular Signaling Cascades01:24

Intracellular Signaling Cascades

53.9K
Once a ligand binds to a receptor, the signal is transmitted through the membrane and into the cytoplasm. The continuation of a signal in this manner is called signal transduction. Signal transduction only occurs with cell-surface receptors, which cannot interact with most components of the cell, such as DNA. Only internal receptors can interact directly with DNA in the nucleus to initiate protein synthesis. When a ligand binds to its receptor, conformational changes occur that affect the...
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Transport Across the Golgi01:26

Transport Across the Golgi

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While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
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What are Second Messengers?01:12

What are Second Messengers?

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Because many receptor binding ligands are hydrophilic, they do not cross the cell membrane and thus their message must be relayed to a second messenger on the inside. There are several second messenger pathways, each with their own way of relaying information. G-protein coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol path is active when the receptor induces phospholipase C to hydrolyze the phospholipid,...
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Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
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STING 生まれつきの免疫 ゴルギから信号を送る

Tomohiko Taguchi1

  • 1Graduate School of Life Sciences, Tohoku University, Sendai, Japan. tomohiko.taguchi.b8@tohoku.ac.jp.

Sub-cellular biochemistry
|February 20, 2026
PubMed
まとめ

インターフェロン遺伝子 (STING) 経路のサイクルGMP-AMP合成酵素 (cGAS) -刺激剤が炎症を誘導する. STINGの活性化には,ER-to-Golgiの輸送が含まれ,COPA症候群に関連した失調があります.

科学分野:

  • 免疫学 免疫学とは
  • 細胞生物学 細胞生物学
  • 分子生物学は分子生物学である.

背景:

  • cGAS-STING経路は,先天性免疫の重要なレギュラーであり,炎症反応を開始するために細胞塩基二鎖DNA (dsDNA) を感知します.
  • アクティベーションには,エンドプラズマ網膜 (ER) からゴルギ装置へのSTING転位が含まれ,TBK1とIRF3.3経由でI型インターフェロン生成につながります.
  • ERとGolgiの間の規制不良の膜密輸は,COPA症候群のような自己炎症性疾患に関与しています.

研究 の 目的:

  • 膜トラフィックのダイナミクスによるcGAS-STING経路活性化の調節を探求する.
  • トランス・ゴルギネットワーク (TGN) でのTBK1/IRF3活性化を制御する分子メカニズムを解明する.

主な方法:

  • 生細胞画像を用いたSTINGの局所化と動態の調査.
  • TGNでタンパク質の相互作用を分析する.
  • COPA症候群の遺伝モデルを使用して,ER-Golgi輸送欠陥を研究する.

主要な成果:

  • ERからゴルギ川へのSTINGの移転は,その活性化に不可欠です.
  • TGNにおける特定の膜密輸事件は,TBK1/IRF3の募集と活性化に極めて重要です.
キーワード:
コレステロール コレステロールIRF3 IRF3 IRF3 IRF3 IRF3 IRF3生まれつきの免疫シグナリングタンパク質のパルミトヨル化.スティング (sting) とは,刺さったもの.TBK1は,TBK1に該当する.トランス・ゴルギ・ネットワーク

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  • ER-Golgi輸送に影響する変異は,STINGシグナル伝達を妨害し,自己炎症に寄与する.

    • 結論:

    • ERとGolgiの間の膜交通は,cGAS-STING経路の重要な規制チェックポイントです.
    • STING媒介のシグナル伝達と関連する膜輸送経路をターゲットにすることは,炎症性疾患と癌の治療の可能性を持っています.