Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

10.0K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.0K
MicroRNAs01:22

MicroRNAs

4.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.1K
MicroRNAs01:22

MicroRNAs

24.4K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.4K
Types of RNA01:20

Types of RNA

10.0K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
10.0K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

12.0K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
12.0K
Mitochondrial Precursor Proteins01:39

Mitochondrial Precursor Proteins

3.8K
Mitochondrial precursors are partially unfolded or loosely folded polypeptide chains. Newly synthesized precursors are inhibited from spontaneously folding into their native conformation by the cytosolic chaperones, heat shock proteins 70 (Hsp70), and mitochondrial import stimulation factors (MSFs). Precursors bound to MSFs are guided to the TOM70-TOM37 receptors, while precursors bound to Hsp70  chaperones are targetted to TOM20-TOM22 receptor complexes.
Most of the mitochondrial...
3.8K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Extracellular Vesicles Mediate Activation and Trafficking of Splenic Immune Cells to the Heart Post-Myocardial Infarction.

bioRxiv : the preprint server for biology·2026
Same author

BK Channels Orchestrate Cardiac Homeostasis Through Mitochondrial Uncoupling Proteins.

bioRxiv : the preprint server for biology·2026
Same author

Eukaryotic Initiation Factor 3F (eIF3F) Regulates the IRES-Mediated Translation of Bcl-xL via Its Interaction with Programmed Cell Death 4 (PDCD4) Protein.

International journal of molecular sciences·2026
Same author

Distribution of microbial carrageenan foraging pathways reveals a widespread latent trait within the ruminant intestinal microbiome.

Nature communications·2026
Same author

Cleavage region organizes the structural architecture of the SINE-derived B2 repressive ribozyme.

Communications biology·2026
Same author

Ion channel and biophysical properties of extracellular vesicles.

The Journal of biological chemistry·2026

関連する実験動画

Updated: Feb 22, 2026

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

1.2K

人間のlncRNARMRPは,DEAD-boxヘリカーズと相互作用し,ミトコンドリア機能を調節する.

Higor Sette Pereira1, Jason Luddu1, Govardhan Reddy Veerareddygari1

  • 1Alberta RNA Research and Training Institute, Department of Chemistry and Biochemistry, University of Lethbridge, Lethbridge, AB T1K 3M4, Canada.

Proceedings of the National Academy of Sciences of the United States of America
|February 20, 2026
PubMed
まとめ

長い非コーディングRNARMRPは,構造的な柔軟性を示し,RNAヘリコゼと相互作用することにより,ミトコンドリアの健康を調節する. このIncRNAは

キーワード:
DEAD-boxヘリカーゼは,デッドボックスヘリカーゼとRMRPRPは,RMRPに設定されています.ロング・ノンコーディングRNAミトコンドリアの機能

さらに関連する動画

Detection of Mitochondria Membrane Potential to Study CLIC4 Knockdown-induced HN4 Cell Apoptosis In Vitro
13:20

Detection of Mitochondria Membrane Potential to Study CLIC4 Knockdown-induced HN4 Cell Apoptosis In Vitro

Published on: July 17, 2018

10.8K
A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

2.4K

関連する実験動画

Last Updated: Feb 22, 2026

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

1.2K
Detection of Mitochondria Membrane Potential to Study CLIC4 Knockdown-induced HN4 Cell Apoptosis In Vitro
13:20

Detection of Mitochondria Membrane Potential to Study CLIC4 Knockdown-induced HN4 Cell Apoptosis In Vitro

Published on: July 17, 2018

10.8K
A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

2.4K

科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • バイオケミストリー バイオケミストリー

背景:

  • 人間のロングノンコーディングRNA (lncRNA) RMRPは疾患に関与しているが,その構造と機能は不明である.
  • RNase MRP コンプレックス コンポーネント RMRP の役割は,このコンプレックスを超えて明らかにする必要があります.

研究 の 目的:

  • RMRPの構造,タンパク質の相互作用,ミトコンドリアの役割を包括的に分析する.
  • ミトコンドリア機能を維持するRMRPの分子メカニズムを理解する.

主な方法:

  • RMRP構造を決定するための小角X線散射 (SAXS)
  • DEAD-box RNAヘリケーズDDX5およびDDX3Xによるタンパク質-RNA相互作用に関する研究.
  • RMRPレベルの変化によるミトコンドリア機能と遺伝子発現の分析.

主要な成果:

  • RMRPは,Mg2+に依存する構造変化を示し,異なる形状を採用します.
  • DDX5とDDX3XヘリゼはRMRPと相互作用し,その局所化と活性に影響を与えます.
  • 減少したRMRPレベルはミトコンドリアの安定性を損ない,脱極化を引き起こし,ROSを増加させます.
  • RMRPは,核にコードされたミトコンドリアタンパク質DNAJC11とNDUFS8.8を調節する.

結論:

  • RMRPは,構造的にダイナミックなlncRNAであり,ミトコンドリアの完全性にとって極めて重要です.
  • RMRPは,特定の遺伝子調節を通じてミトコンドリアの健康を維持するためにRNAヘリコゼと連携します.
  • 発見は,RMRPに関連する疾患と潜在的な治療戦略の洞察を提供します.