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Updated: Feb 22, 2026

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach
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Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

Published on: September 26, 2019

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1H NMRと独立した成分分析を用いて,粗製ヘパリンにおけるコンドロイトン硫酸とダーマタン硫酸の同時定量化

René Burger1, Xuan Tung Do1, Bernd W K Diehl2

  • 1Bonn-Rhein-Sieg University of Applied Sciences, Department of Natural Sciences, von-Liebig-Straße 20, 53359 Rheinbach, Germany.

Journal of pharmaceutical and biomedical analysis
|February 20, 2026
PubMed
まとめ

ヘパリン中のコンドロイチン硫酸 (CS) とダーマタン硫酸 (DS) の不純物質を定量化することは,安全性にとって極めて重要です. 1H NMRと独立成分分析 (ICA) の新しい方法は,ヘパリン品質管理のための従来のアッセイに対するより速く,よりシンプルな代替手段を提供します.

キーワード:
1時間NMRによる.ベンチトップのNMRはケミオメトリクス 化学測定法コンドロイトン (chondroitin) とはダーマタン (Dermatan) とは ダーマタン (Dermatan) とは ダーマタン (Dermatan) とはヘパリン (Heparin) とはICAICAICAICAICAICAICAICAICAICAICAICAICAICAICAICAICAICAICAICAICAICA

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2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
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2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
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¹H NMR Signal Integration: Overview00:58

¹H NMR Signal Integration: Overview

The intensity of a signal, which can be represented by the area under the peak, depends on the number of protons contributing to that signal. The area under each peak is shown as a vertical line called an integral, with the integral value listed under it, as seen in the proton NMR spectrum of benzyl acetate. Each integral value is divided by the smallest integral value to obtain the ratio of the number of protons producing each signal. The ratio reveals the relative number of protons and not...
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¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
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¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
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