食品用途のサブクリティカル水解: 肉タンパク質の温度依存変換と生物活性
Jong Won Lee1, SangYoon Lee1, Geun-Pyo Hong2
1Department of Food Science & Biotechnology, and Carbohydrate Bioproduct Research Center, Sejong University, Seoul, South Korea.
NPJ science of food
|February 20, 2026
まとめ
サブクリティカル・ウォーター (SW) 水解により,豚肉のタンパク質を生物活性成分に効果的に変換します. 250°Cの最適な条件では,酵素水解 (EH) を上回る高い回復率,強力な生物活性,そして望ましい味が得られます.
科学分野:
- 食品科学 食品科学について
- バイオテクノロジー バイオテクノロジー
- タンパク質化学 タンパク質化学
背景:
- 肉タンパク質の加工には,しばしば酵素性水解 (EH) が含まれる.
- 肉のタンパク質の価値を高めるために,代替的で効率的な方法を探求することは極めて重要です.
- サブクリティカルウォーター (SW) 水解は,潜在的にグリーンなアプローチを提供します.
研究 の 目的:
- 豚骨のタンパク質を生物活性成分に変換するための亜臨界水 (SW) 水解を評価する.
- SW水解と酵素水解 (EH) を比較して,タンパク質の回復と生物活性について.
- 高価な肉タンパク質の成分を生産するための最適なSW条件を決定する.
主な方法:
- 豚 loinは,100°Cから300°Cまでの温度で,亜臨界水 (SW) 水解を施した.
- 水解剤は,タンパク質の回復,自由アミノ群の含有量,生物活性 (抗酸化,ACE阻害),細胞毒性,および感覚的属性 (苦味,ウマミ) について分析されました.
- 結果は,酵素性水解 (EH) により得られた結果と比較されました.
主要な成果:
- 200°C以上のSW治療は,EHと比較して,タンパク質の回復と自由アミノ群の含有量を大幅に改善しました.
- 最高のタンパク質回収は,SW経由で300°Cで達成されました.
- 250°C以上のSWからの水解剤は,強い抗酸化作用とACE阻害作用を示したが,細胞毒性は示されなかった.
- しかし,250°C以上のSWは,苦味を増加させ,プロバイオティクスの成長を阻害しました.
- 250°C未満のSW治療は,EHと比較して,苦味を軽減し,ウマミの強度を高めました.
結論:
- サブクリティカル水 (SW) 水解は,肉タンパク質の変換に有望な方法である.
- 250°Cでの最適なSW処理は,高いタンパク質回収率,強力な生物活性,そして好ましい味のプロフィールとのバランスをとります.
- このアプローチは,高価値の食品原料に豚の loin タンパク質の価値を高めるための実行可能な戦略を提供します.
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