Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

9.8K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
9.8K
Regulated Protein Degradation02:58

Regulated Protein Degradation

9.0K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
9.0K
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

3.1K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
3.1K
The Unfolded Protein Response01:37

The Unfolded Protein Response

6.5K
The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...
6.5K
Phosphorylation01:02

Phosphorylation

54.7K
The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
54.7K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.9K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.9K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Dual regulation of RNase P subunit Rpp30 by an acetyltransferase and E3 ligase in rice immunity.

Plant physiology·2026
Same author

A Conserved Magnaporthe oryzae Effector Counteracts the Rice Ubiquitin-Proteasome System by Disrupting the E2 Function to Suppress Immunity.

Plant biotechnology journal·2026
Same author

E3 ubiquitin ligase-mediated degradation of Rab GTPase suppresses an MAPKK and activates immunity in rice.

Journal of integrative plant biology·2026
Same author

Balancing rice blast resistance and growth through suppression of the E3 ubiquitin ligase OsRING80.

Plant physiology·2025
Same author

Phosphorylation and ubiquitination synergistically promote the degradation of OsRbohB to modulate rice immunity.

The Plant cell·2025
Same author

Natural variation in SBRR1 shows high potential for sheath blight resistance breeding in rice.

Nature genetics·2025

関連する実験動画

Updated: Feb 23, 2026

In-vitro Reconstitution of Bacterial Ubiquitination and VCP/p97-mediated Elimination
07:58

In-vitro Reconstitution of Bacterial Ubiquitination and VCP/p97-mediated Elimination

Published on: January 2, 2026

416

リン酸化に依存するユビキチネーションスイッチは,キチンの知覚に対する核免疫再プログラムをオーケストラします.

Chongyang Zhang1,2,3, Pavinee Suttiviriya2, Ruyi Wang1

  • 1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

Nature communications
|February 21, 2026
PubMed
まとめ

米14-3-3タンパク質のOsGF14fとOsGF14cは,ライスブラスト菌に対する耐性を高めます. チチンの知覚は,これらのタンパク質を安定させる経路を誘発し,Magnaporthe oryzaeに対する植物免疫を強化します.

さらに関連する動画

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
11:36

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones

Published on: July 25, 2019

11.5K
Identification of Post-translational Modifications of Plant Protein Complexes
10:07

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

24.7K

関連する実験動画

Last Updated: Feb 23, 2026

In-vitro Reconstitution of Bacterial Ubiquitination and VCP/p97-mediated Elimination
07:58

In-vitro Reconstitution of Bacterial Ubiquitination and VCP/p97-mediated Elimination

Published on: January 2, 2026

416
In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
11:36

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones

Published on: July 25, 2019

11.5K
Identification of Post-translational Modifications of Plant Protein Complexes
10:07

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

24.7K

科学分野:

  • 植物生物学 植物生物学
  • 分子植物病理学 分子植物病理学
  • バイオケミストリー バイオケミストリー

背景:

  • 14-3-3タンパク質は,植物の成長とストレス反応に不可欠です.
  • 植物免疫におけるそれらの特定の役割と規制メカニズムは十分に理解されていません.

研究 の 目的:

  • マグナポルテ・オライゼ (Magnaporthe oryzae) に対する免疫における米14-3-3タンパク質の機能を調査する.
  • 米植物の免疫を制御する規制メカニズムを明らかにする.

主な方法:

  • 米におけるOsGF14fとOsGF14cの機能を調査した.
  • タンパク質の分解におけるE3リガゼOsPUB20の役割を分析した.
  • チチンの知覚とOsRLCK185のリン酸化の効果を研究した.
  • 顕微鏡検査と生化学分析を用いて,タンパク質の局所化と相互作用を調べた.

主要な成果:

  • OsGF14fとOsGF14cは,マグナポルテオライザ (Magnaporthe oryzae) に対する米の耐性を増強する.
  • OsPUB20は,OsGF14f/OsGF14cを分解し,免疫を否定的に調節する標的となる.
  • チチンの知覚は,OsRLCK185を活性化し,OsPUB20をリン酸化し,安定させ,それによって耐性を高める.
  • OsGF14fは核に移動し,負の調節器OsWRKY42.4を分解する.

結論:

  • 酸化に依存する新しいユビキチネーションスイッチが,米の免疫を調節する.
  • このメカニズムは,細胞表面キチンの知覚と核防衛反応を結びつける.
  • 発見は,植物免疫信号伝達経路と作物の改善のための潜在的なターゲットについての洞察を提供します.