Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Eukaryotic Compartmentalizations01:46

Eukaryotic Compartmentalizations

One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal cells...
Eukaryotic Compartmentalization01:46

Eukaryotic Compartmentalization

One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal cells...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Eukaryotic Compartmentalization01:37

Eukaryotic Compartmentalization

One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal cells...
Entropy within the Cell01:22

Entropy within the Cell

A living cell's primary tasks of obtaining, transforming, and using energy to do work may seem simple. However, the second law of thermodynamics explains why these tasks are harder than they appear. None of the energy transfers in the universe are completely efficient. In every energy transfer, some amount of energy is lost in a form that is unusable. In most cases, this form is heat energy. Thermodynamically, heat energy is defined as the energy transferred from one system to another that is...
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Aerobic oxidative condensation for the synthesis of phenoxazinone and phenazine derivatives catalyzed by an iron-porphyrin complex with a pendant imidazole ligand.

RSC advances·2026
Same author

Psychological profiles of mindfulness and tourist fatigue: a latent profile analysis in a digital travel context.

Scientific reports·2026
Same author

Investigating the crystalline structure and structural heterogeneity of starch granules using polarization-based quantitative phase microscopy.

Food chemistry·2026
Same author

Micropeptide YG-6 encoded by exosomal LINC01123 derived from highly migratory ovarian cancer cells promotes tumor progression.

Molecular cancer·2026
Same author

Spacer cations: molecular switches for 2D and 2D/3D perovskite photovoltaics.

Nanoscale·2026
Same author

MFST-GCN: A Sleep Stage Classification Method Based on Multi-Feature Spatio-Temporal Graph Convolutional Network.

Brain sciences·2026
Same journal

Kat5 deficiency in alveolar type II cells licenses STAT6-driven glycolytic reprogramming and pulmonary fibrosis.

Nature communications·2026
Same journal

Continuous nonthermal slab gap formed by progressive tearing beneath Northeast Asia.

Nature communications·2026
Same journal

Zeolitic isolated protonic acid sites-mediated NH<sub>3</sub> storage for robust NO<sub>x</sub> removal.

Nature communications·2026
Same journal

Coaxially nested component with asymmetric fiber resonant cavity and separation membrane for gaseous and dissolved gases detection.

Nature communications·2026
Same journal

Near-unity charge readout signal in a nonlinear resonator without matching the sensor dissipation.

Nature communications·2026
Same journal

Prokaryotic Schlafen proteins cleave tRNAs during type III CRISPR immunity.

Nature communications·2026
関連記事をすべて見る

関連する実験動画

Updated: May 11, 2026

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.5K

空間的な混乱-seq: 完ぺきな組織構造内の単細胞機能ゲノミクス.

Kimberle Shen1, Wan Yi Seow1, Choong Tat Keng1

  • 1Genome Institute of Singapore (GIS), Agency for Science, Technology and Research (A*STAR), 60 Biopolis Street, Genome, Singapore, Republic of Singapore.

Nature communications
|February 21, 2026
PubMed
まとめ
この要約は機械生成です。

Spatial Perturb-Seqは,無傷の組織内の複数の遺伝子のCRISPRスクリーニングを in vivoで可能にします. この方法は,遺伝子ノックアウトの細胞特異的およびマイクロ環境的影響を明らかにし,神経変性疾患の研究を支援します.

さらに関連する動画

Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq
10:22

Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq

Published on: October 31, 2025

631
Mining Spatial Transcriptomics Datasets using DeepSpaceDB
10:16

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Published on: September 5, 2025

856

関連する実験動画

Last Updated: May 11, 2026

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.5K
Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq
10:22

Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq

Published on: October 31, 2025

631
Mining Spatial Transcriptomics Datasets using DeepSpaceDB
10:16

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Published on: September 5, 2025

856

科学分野:

  • ゲノミクスゲノミクスとは
  • 神経科学は神経科学である.
  • 分子生物学は分子生物学である.

背景:

  • 複雑な組織における遺伝子の機能を理解することは,疾患の研究において極めて重要です.
  • 既存の方法は,分析中に空間的な文脈と細胞表現を維持するためにしばしば苦労します.
  • 神経変性疾患は,脳内の複雑な遺伝子と環境の相互作用を伴う.

研究 の 目的:

  • 健全な組織における高通量機能的遺伝子スクリーニングのための新しい in vivo CRISPR テクノロジーを開発する.
  • 神経退行性疾患に関連する遺伝子ノックアウトの細胞自律性および細胞内マイクロ環境効果を調査する.
  • ニューロン細胞間通信に関与する候補遺伝子を特定する.

主な方法:

  • Spatial Perturb-Seq:無傷組織内の単細胞内の複数の遺伝子を尋問するためのインビボCRISPR技術.
  • シーケンシングベースの空間技術と探査ベースの空間技術との互換性.
  • ネズミの脳における神経変性疾患のノックアウトリスク遺伝子の適用.

主要な成果:

  • 複数の遺伝子を in situ および in vivo で機能的にスクリーニングするために,Spatial Perturb-Seq を成功裏に適用しました.
  • 細胞のタイプ表現を歪める可能性のある細胞処理ステップをバイパスし,組織構造を保存します.
  • 遺伝子ノックアウトの細胞内および細胞間効果の両方を特定しました.
  • 空間的に無傷なマウスの脳内の細胞自律的および細胞-細胞の微環境効果を明らかにした.
  • 制御不能なニューロン細胞間通信経路の基礎となる候補遺伝子を特定しました.

結論:

  • Spatial Perturb-Seqは,無傷の組織における in vivo 機能的ゲノミクスのための強力なツールです.
  • この技術は,神経変性疾患のモデルにおける複雑な遺伝子相互作用とマイクロ環境の影響を解明します.
  • このアプローチは,神経細胞のコミュニケーションの研究を進め,潜在的な治療標的を特定します.