pSi-SU-8-GQDヘテロ構造に基づくバイオセンサーによるデュアルシグナル増幅戦略を用いたウイルス検出の強化

DNA microarray technology requires optimizing substrate properties and DNA labeling strategies for enhanced detection. This study introduces an improved biosensor platform utilizing nanoporous silicon (pSi) substrates with a glycidyl ether of bisphenol A (SU-8) polymer coating, decorated with graphene quantum dots (GQDs), to amplify fluorescent signals. We compared two methods for obtaining labeled single-stranded DNA: Lambda exonuclease digestion of phosphorylated PCR products and asymmetric PCR (aPCR) with Cy5-labeled nested primers. Our findings demonstrate that pSi biochips with 7 nm pores covered by thicker SU-8 layers (700-750 nm) provide 52% higher signal relative to the standard deviation. The aPCR labeling method demonstrated superior performance, yielding significantly stronger signals -4.06 mean signal intensity and a standard deviation of 0.16. Decorating the SU-8 coating with 6 nm GQDs further amplified the signal intensity by 11%. These findings highlight the need to optimize DNA labeling and utilize nanostructured heterostructures for enhanced microarray performance, with implications for personalized medicine and diagnostics.