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Updated: Feb 24, 2026

Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures

Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures

Published on: March 20, 2019

神経分化における脂質代謝を支持するためのオルガネラコミュニケーションネットワークの再配線

Maria Clara Zanellati, Zachary Coman, Disha Bhowmik

bioRxiv : the preprint server for biology

|February 23, 2026

Abstract:

Cell fate transitions require coordinated remodeling of intracellular organelles, but how the organelle interactome rewires during neurogenesis remains unclear. Here we combine multispectral imaging with quantitative organelle signature analysis to simultaneously map eight organelles at single-cell resolution as human induced pluripotent stem cells (iPSCs) differentiate into forebrain-like neurons. We find compartment and time-specific rescaling of organelles and a progressive increase in higher-order membrane contacts, with mitochondria emerging as an early interaction hub. Later, endoplasmic reticulum (ER)-organelle contacts dominate with ER-peroxisome contacts promoting plasmalogen biosynthesis, membrane homeostasis and synapse formation. Disrupting this contact impairs plasmalogen production, synaptic organization, and neuronal activity, identifying the ER-peroxisome axis as a key regulator of neuronal maturation.

キーワード:

神経発生オルガネラ小胞体ペルオキシソームシナプス形成脂質代謝神経分化

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Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum

Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum

Published on: January 22, 2019

Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions

Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions

Published on: November 9, 2020

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