ラクトバチルス属複合体のアレルゲン性緩和のためのインシリコオミクス調査
Zahra Bigdeli1, Ali Molaei2, Nassim Rahmani3
1Department of Systems Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Background:
Among the useful methods of immune response modulation, probiotics can be included. A delicate balance in the microbiome is of extreme importance for immune homeostasis, the composition of which is influenced by lifestyle and dietary habits. The aim of this study was to identify Lactobacillus strains with potential allergenicity-mitigating properties through comprehensive in silico analysis of their genomes and proteomes. Abrogation in such a balance may lead to the development of food allergy. So far, certain components in probiotics, including immunosuppressive motifs or metabolites like 17,18-epoxy eicosatetraenoic acid (17,18 EpETE), seem to have the potential to reduce prevalence. However, some bacterial proteins may be allergenic or may induce cross-reactivity.
Methods:
We performed an in silico study over the 161 reference genomes and proteomes of Lactobacillus. We analyzed the presence of Nucleotide immunosuppressive motifs (NISM)s, calculated the per million frequency of bases, and checked for the presence of the CYP450 enzymes/stable allergen epitopes. This was evaluated using various bioinformatics tools like Uniprot proteomes, NCBI genomes, AlgPred, PeptideCutter, EMBOSS, and MAST.
Results:
Our analysis identified Latilactobacillus sakei as a promising candidate, based on its genomic and proteomic features, for mitigating allergenicity.
Conclusions:
Latilactobacillus sakei might be a useful candidate for nutritional allergy management and related conditions. However, this potential requires verification through further experimental studies.
