PI(3)P依存性Klp98Aリクルートメントは、Rab4を介した発達シナプスリモデリングの基盤となるエンドソームフラックスを調節する

Rab4 GTPase, essential for endosomal sorting and trafficking, is implicated in synaptic atrophy and dementia. To uncover the underlying mechanism, we studied the correlation between Rab4 vesicle transport in axons and episodic remodeling of synapses in the central nervous system (CNS) of Drosophila larvae. We found that synapse-bound traffic and presynaptic enrichment of Rab4 vesicles increase during the programmed, transient contraction of gross synapse density in the ventral neuropil region at a specific larval stage. This reduction in the gross synapse density coincides with the episodic activation of insulin and Vps34-mediated signaling, which elevates phosphatidylinositol-3-phosphate levels on Rab4 vesicles. The presence of this phospholipid on Rab4-associated vesicles recruits a PX-domain-containing motor protein, Klp98A, accelerating their synapse-directed traffic. This, in turn, increases presynaptic enrichment of Rab4 during the developmentally programmed synapse contraction phase. Our findings elucidate the molecular mechanism that regulates developmental synaptic plasticity in the CNS via Vps34-depedent regulation of directed axonal transport of endosomes.