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関連する概念動画

Mechanisms of Membrane-bending01:15

Mechanisms of Membrane-bending

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The living membranes are flexible due to their fluid mosaic nature; however, their bending into different shapes is an active process regulated by specific lipids and proteins. The membrane bending can be transient as seen in vesicles or stable for a long time as in microvilli. Cells regulate the size, location, and duration of the membrane curvature.
Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...
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The Supercomplexes in the Crista Membrane01:41

The Supercomplexes in the Crista Membrane

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The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
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Protein Translocation Machinery on the ER Membrane01:28

Protein Translocation Machinery on the ER Membrane

7.1K
The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
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Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

4.2K
Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with...
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SNAREs and Membrane Fusion01:43

SNAREs and Membrane Fusion

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Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
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Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

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Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
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関連する実験動画

Updated: Feb 24, 2026

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions
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タンパク質誘発性膜ひずみがスーパー複合体形成を駆動する

Maximilian C Pöverlein1, Alexander Jussupow1, Hyunho Kim1

  • 1Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.

eLife
|February 23, 2026
PubMed
まとめ
この要約は機械生成です。

ミトコンドリア内の電子伝達系タンパク質のスーパー複合体は膜ひずみを軽減し、ATP産生を改善します。この組み立ては、混雑した細胞環境での呼吸機能を最適化します。

キーワード:
生体エネルギー学分子物理学分子動力学タンパク質–膜相互作用呼吸複合体構造生物学スーパー複合体

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Pulling Membrane Nanotubes from Giant Unilamellar Vesicles
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Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies
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Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies

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関連する実験動画

Last Updated: Feb 24, 2026

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions
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Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

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Pulling Membrane Nanotubes from Giant Unilamellar Vesicles
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Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies
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科学分野:

  • ミトコンドリア生物学
  • 生物物理学
  • 分子動力学

背景:

  • ミトコンドリア膜には、酸化的リン酸化（OXPHOS）によるATP合成に不可欠な電子伝達系（ETC）が含まれています。
  • ETCのタンパク質はスーパー複合体（SC）に組み立てられますが、その機能的重要性は議論されています。

研究 の 目的:

  • 哺乳類のETCスーパー複合体、特にI/III2 SCの機能的役割を調査すること。
  • SC形成がミトコンドリア膜の特性とタンパク質の動力学にどのように影響するかを理解すること。

主な方法:

  • 大規模な原子論的および粗視化分子シミュレーション。
  • クライオ電子顕微鏡データの分析。
  • 統計的および速度論的モデリング。

主要な成果:

  • 哺乳類のI/III2 SC形成は、局所的な膜厚を変化させることにより、内側ミトコンドリア膜のひずみを軽減します。
  • SCアセンブリは、スーパー複合体の周りのカルジオリピンとキノンの蓄積を促進します。
  • スーパー複合体形成は、個々のETCタンパク質の全体的な運動に影響を与えます。

結論:

  • 分子の混雑と膜のひずみは、SC形成の熱力学的駆動力として作用します。
  • SCアセンブリは、制約された条件下で混雑した膜の呼吸フラックスを強化する可能性があります。