関連する実験動画

Updated: May 5, 2026

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling
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RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling

Published on: April 11, 2015

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軸索mRNAの分離と定量:多孔質メンブレンインサートとRTddPCRの使用

Shruti Ghumra1, Manasi Agrawal1, Meghal Desai1

  • 1Department of Biological Sciences, Rutgers University- Newark.

Journal of visualized experiments : JoVE
|February 23, 2026
PubMed
まとめ

No abstract available in PubMed .

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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