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Updated: Jun 20, 2026

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
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DNP-NMR緩和測定法を用いたリガンド-膜相互作用の定量化

Chang Qi1, Nirmalya Pradhan1, Christian Hilty1

  • 1Chemistry Department, Texas A&M University, College Station, Texas 77843, United States.

Analytical chemistry
|February 24, 2026
PubMed
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No abstract available in PubMed .

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¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...