MACSimaイメージングサイクリック染色技術を用いたマウス二次リンパ器官における胚中心の空間的プロファイリング

We developed a 50-plex imaging protocol using the MACSima platform to characterize the microarchitecture of germinal centers in secondary lymphoid tissues. This workflow combines tissue processing, automated cyclic imaging, image preprocessing, and a new analysis method that detects morphological changes in irregularly shaped stromal cells.