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High-Resolution Mass Spectrometry (HRMS)01:15

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The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
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The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
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The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
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Imaging Corrosion at the Metal-Paint Interface Using Time-of-Flight Secondary Ion Mass Spectrometry
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高分解能顕微鏡モード二次イオン質量分析イメージング

Yifeng Jia1, Maria Elena Castellani1,2, Kieran Cheung1

  • 1The Department of Chemistry, The Chemistry Research Laboratory, The University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, United Kingdom.

Analytical chemistry
|February 24, 2026
PubMed
まとめ

新しい二次イオン質量分析（SIMS）装置は、ハイスループットイメージングを提供します。この高度な顕微鏡は、マウスの脳組織のような生物学的サンプルを分析するために、高い質量分解能と空間分解能を備えています。

キーワード:
二次イオン質量分析イメージングハイスループット質量分解能空間分解能生物組織顕微鏡

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科学分野:

  • 分析化学;生物物理学;材料科学

背景:

  • 二次イオン質量分析（SIMS）は強力な表面分析技術です。;ハイスループットイメージング機能は、大きな生物学的サンプルの分析に不可欠です。;既存のSIMS装置は、速度または分解能に限界がある場合があります。

研究 の 目的:

  • 新しいSIMS顕微鏡モードイメージング装置を開発すること。;ハイスループット、質量分解能、空間分解能を達成すること。;生物組織分析における装置の有用性を実証すること。

主な方法:

  • 飛行時間型質量分析とパルスイオン抽出の組み合わせ。;高速シンチレータスクリーンを備えたイオンイメージング検出器の使用。;質量および空間分解能のための装置パラメータの最適化。

主要な成果:

  • 質量分解能は約2000（検出器の改善により最大約6900）を達成しました。;空間分解能は5μm未満を得ました。;マウス脳組織内の原子および分子イオン種を数分以内にイメージングすることに成功しました。

結論:

  • 開発されたSIMS装置は、生物学的サンプルの迅速かつ高解像度のイメージングを可能にします。;ハイスループットを必要とする幅広いアプリケーションに適しています。;複雑な組織における生物学的に関連のあるイオンの分析の可能性を示しています。