関連する実験動画

Updated: Jul 1, 2026

Scanning Electron Microscopy SEM Protocols for Problematic Plant, Oomycete, and Fungal Samples

Scanning Electron Microscopy SEM Protocols for Problematic Plant, Oomycete, and Fungal Samples

Published on: February 3, 2017

含水率変動下における珪藻殻の曲率補正SEM形態計測：投影から真の表面へ

Izabela Zglobicka¹, Maciej Łojkowski¹, Ewa Borucinska¹

¹Faculty of Mechanical Engineering, Bialystok University of Technology, Bialystok, Poland.

Journal of microscopy

|February 24, 2026

まとめ

走査型電子顕微鏡（SEM）と共焦点顕微鏡を組み合わせることで、正確な珪藻殻形態計測を実現する。この手法は、SEM投影歪みと乾燥による細孔収縮を補正し、信頼性の高い細孔サイズ評価を提供する。

背景:

珪藻殻は、走査型電子顕微鏡（SEM）で広範に研究されている複雑なサブミクロンシリカ構造を持っている。
SEMイメージングは2D投影を提供し、珪藻殻の3D形態と次元を歪める。
SEMのサンプル調製は通常脱水を含み、これは細孔収縮のような構造変化を引き起こす可能性がある。

結論:

開発された曲率認識補正モデルは、珪藻殻の正確な形態計測データを提供する。
含水/脱水イメージングと補正されたSEMデータを組み合わせることで、細孔サイズ評価のための信頼性の高いフレームワークが提供される。
この定量的フレームワークは、分類学、生物モニタリング、生体模倣設計を含む、正確な生体由来シリカ構造分析を必要とする様々な分野に広く適用可能である。

キーワード:

SEM形態計測生体模倣材料曲率補正珪藻含水状態

さらに関連する動画

Measuring and Modeling Contractile Drying in Human Stratum Corneum

Measuring and Modeling Contractile Drying in Human Stratum Corneum

Published on: March 1, 2017

Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins

Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins

Published on: August 17, 2022

関連する概念動画

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

Scanning Electron Microscopy

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...

Preparation of Samples for Electron Microscopy

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...