フェンプロパトリンの蛍光試験紙検出のための遺伝子工学技術によるナノボディの部位指向性修飾

The lateral flow immunochromatography assay (LFIA) offers a rapid and on-site method for food safety. However, the current LFIA shows a limitation due to the random binding between the label and antibodies resulting in target recognition sites on the antibody being occupied yielding false-negative outcomes. To address these issues, this study utilized genetic engineering technology to integrate the super folded green fluorescent protein (SGFP) gene into the sequence of a specific nanobody for fenpropathrin, a Nb-SGFP fluorescent probe, realizing site-directed modification to avoid the block of recognition sites. Meanwhile, it also minimizes potential antibody performance issues during the process that may affect antibody affinity. Based on this, a fluorescent strip detection method was established, providing a new strategy for efficient and convenient detection of fenpropathrin. The detection limit of the method was 1.2 ng mL^-1, which adequately met the sensitivity requirements for fenpropathrin detection. To validate its accuracy, the fluorescent test strip method was compared with GC-MS/MS analysis. The strong correlation between the two methods confirms that our approach enables rapid detection of fenpropathrin residues in fruits and vegetables.