陽電子放出断層撮影（PET）トレーサー中のKryptofix 2.2.2含有量検出のためのポストカラム誘導体化高速液体クロマトグラフィー-紫外吸光光度法（HPLC-UV）

A post-column derivatization high-performance liquid chromatography-ultraviolet (HPLC-UV) method was developed to determine Kryptofix 2.2.2 (K2.2.2) in PET tracers. This method utilizes the specific complexation between K2.2.2 and Pb²⁺, forming a stable 1:1 host-guest complex. The complexation produces a bathochromic shift in UV absorption maximum (from 210 nm to 254 nm), enabling sensitive detection. This approach addresses the limitations of poor performance and narrow applicability associated with direct single-method detection of K2.2.2. The chromatographic conditions were optimized (column flow rate: 0.7 mL·min^-1; derivatization reagent flow rate: 0.7 mL·min^-1; Pb²⁺ concentration: 50 μg·mL^-1) and the method was validated. The results exhibited excellent linearity (1-100 μg·mL^-1, r² > 0.999), high reproducibility (relative standard deviation (RSD) < 5%), and a low detection limit (0.5 μg·mL^-1). The method successfully quantified K2.2.2 in [¹⁸F]FDG, [¹⁸F]AV45 and [¹⁸F]DPA714, achieving spiked recoveries of 89%-105%. Additionally, the method remained unaffected by interference from common sample components such as sodium chloride and sodium ascorbate. This method provides a novel and efficient quality control tool for the simultaneous analysis of radiochemical purity and K2.2.2 content in diverse PET tracers.