3Dプリント多孔質チタンインプラントのキュルクミンおよび間葉系幹細胞由来因子-1によるオンデマンドデリバリーによる骨結合改善

Porous titanium (Ti) implants manufactured via three-dimensional (3D) printing hold considerable promise in the reconstruction of bone defects. However, their osseointegration capacity requires enhancement, and existing techniques remain insufficient. The biofunctionalization of titanium implants-transforming their surface from biologically passive to osteoactive-has emerged as a paramount objective in implantology research. To improve osseointegration of 3D-printed porous Ti implants, this study proposes a novel cell-guided drug delivery system that enables cell recruitment, osteogenic differentiation, and local immunomodulation. Inspired by natural bone osteogenesis, an on-demand delivery system co-loading curcumin (Cur) and stromal cell-derived factor-1 (SDF-1) was designed. SDF-1 and Cur-loaded polycaprolactone (PCL) microspheres (Cur@PCL) were immobilized into the implant pores via a double-crosslinked alginate-chitosan hydrogel coating. The system's effects on osseointegration and immunomodulation were evaluated both in vitro and in vivo. Experiments demonstrated that the system enhanced early-stage cell recruitment via SDF-1 release, priming target cells for Cur activity. Subsequent Cur release induced M2 macrophage polarization and osteogenic differentiation. Furthermore, the SDF-1/Cur synergy potentiated implant osseointegration. In summary, this multifunctional porous implant integrated with a drug delivery system holds clinical potential for patients requiring bone reconstruction with low bone mass.