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関連する概念動画

Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

7.9K
Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
7.9K
Nuclear Protein Sorting01:34

Nuclear Protein Sorting

6.5K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
6.5K
Directionality of Nuclear Transport01:42

Directionality of Nuclear Transport

4.7K
Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of  Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...
4.7K
Nuclear Export01:42

Nuclear Export

5.1K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
5.1K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

3.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.4K
The Nucleosome Core Particle01:12

The Nucleosome Core Particle

2.5K
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
2.5K

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関連する実験動画

Updated: Feb 26, 2026

In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays
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In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays

Published on: December 29, 2021

3.3K

ヒストン核内導入とその先：インポルチンの多機能的役割

Natalia Bernardes1, Yuh Min Chook1

  • 1Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.

BioEssays : news and reviews in molecular, cellular and developmental biology
|February 25, 2026
PubMed
まとめ
この要約は機械生成です。

核内導入受容体であるインポルチンは、ヒストンの輸送とフォールディングに不可欠です。最近の研究では、ヒストンを安定化させ、ヌクレオソームアセンブリへの送達を促進するこれらの二重の役割が明らかになっています。

キーワード:
ヒストン核内導入インポルチンヌクレオソームアセンブリ分子シャペロンクロマチン生物学

さらに関連する動画

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
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Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes

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Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
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Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

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関連する実験動画

Last Updated: Feb 26, 2026

In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays
08:16

In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays

Published on: December 29, 2021

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Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
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Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes

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Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
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Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

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科学分野:

  • 分子生物学、細胞生物学、クロマチン生物学

背景:

  • ヒストンおよびクロマチン調節因子の核内導入は、DNAプロセスに不可欠です。ヒストンは、その塩基性および凝集しやすい性質のため、適切なフォールディングとクロマチン沈着にシャペロンと導入受容体を必要とします。

研究 の 目的:

  • コアおよびリンカーヒストンの導入とシャペロニングのメカニズムに関する最近の進歩をレビューすること。特定のインポルチンがヒストンの核内輸送と安定化において果たす役割を解明すること。

主な方法:

  • ヒストン-インポルチン相互作用を調査するために、構造的および生化学的研究が用いられました。インポルチン4/Kap123、インポルチン9/Kap114、およびインポルチンβ-インポルチン7ヘテロ二量体の分析。

主要な成果:

  • インポルチン4/Kap123は、ASF1と協働して、H3-H4ヘテロ二量体の輸送因子およびシャペロンとして機能します。インポルチン9/Kap114は、Nap1と協働して、RanGTP非依存的なメカニズムを介してH2A-H2Bヘテロ二量体を導入します。インポルチンβ-インポルチン7は、リンカーヒストンH1の導入を媒介し、インポルチンの多機能的な役割を強調しています。

結論:

  • インポルチンは多機能であり、ヒストンの安定化、保護、核内導入、およびヌクレオソームアセンブリへの送達をカップリングします。ヒストントラフィッキングおよびヌクレオソームアセンブリにおける二次インポルチン、ヒストン修飾、およびシャペロンダイナミクスに関するさらなる研究が必要です。