乳牛における分娩時の熱ストレスの影響を受けた細胞外小胞の特性評価

Extracellular vesicles (EV) are recognized as important mediators of cell-cell communication, capable of transferring various cargo between cells. Thus, EV are potential biomarkers of health status in heat-stressed cows. We hypothesized that heat stress during late gestation would alter the protein profile of EV at parturition in dairy cows. To test this, maternal plasma samples were collected within 2 h after parturition (n = 16-17/treatment) from cows exposed to late-gestation heat stress (HT) or an active cooling system (CL). The EV were isolated from plasma via single-step size exclusion chromatography. Preparations were checked for quality by transmission electronic microscopy and then quantified by nanoparticle tracking analysis before proteomic analysis. High-resolution MS was performed, and a two-sided, unpaired Welch's t-test was done on proteins showing at least 3 valid values in one group and at least 70% of valid values in the other group using log2 (label-free quantification intensity). The significance threshold was P ≤ 0.059, and proteins with a log2 fold change (CL/HT) above 1.2 or below 0.66 were considered significantly affected. Potential EV biomarkers from bovine plasma were detected, including several proteins significantly affected when comparing CL and HT. We noted more abundance in the CL group of laminin subunit gamma 1, α 2, and β 1; transferrin receptor protein 1; collagen IV; fibrinogen α chain, gamma-B chain, and β chain; von Willebrand factor; and SPN protein. Furthermore, in the CL group, the less abundant proteins included immunoglobulin domain lambda and heavy chains; HGF activator; protein HP-20 homolog; trafficking from endoplasmic reticulum to Golgi regulator (TFG) protein; amine oxidase 3; and collectin member 10. These findings suggest that these EV may play a role in modulating maternal metabolism and immune adaptations at the time of parturition within the maternal circulation. In addition, late-gestation heat stress alters the secretion of specific proteins that are involved in adaptations related to parturition. Further investigation is needed to better understand the functionality of EV during late gestation and the impact of heat stress on the molecules they carry.