トウモロコシにおけるBSA-seqおよびトランスクリプトーム解析による穂柄長に影響を与えるQTLおよび新規候補遺伝子の同定

Hongzhou An¹, Kuiying Li¹, Xiaolan Liu²

¹Hebei Key Laboratory of Crop Genetics and Breeding, Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, China.

Frontiers in plant science

|February 25, 2026

要旨

Introduction:

The ear shank, a short branch connecting the stalk and the ear, represented a key agronomic trait influenced both yield and plant architecture in maize, yet the molecular mechanism remained not fully understood.

Methods:

In this study, BSA-seq was performed using an F₂ population for two extreme bulks derived from the cross between WL134 and L135. Additionally, transcriptomic analysis and gene annotation were carried out to refine the association interval of ear shank length and identify crucial genes.

Results And Discussion:

A total of 14 QTLs for ear shank length were detected, which included 334 non-synonymous mutants, synonymous mutants and frameshift mutant genes. Among these loci, five were known to be associated with ear shank length, while nine were newly identified. 3,460 differentially expressed genes (DEGs) were screened through RNA-seq analysis of the ear shank at the silking stage in both parents. Thirteen new candidate genes were identified through the combined analysis of BSA-seq and RNA-seq, as well as gene function annotation and gene expression analysis. Based on functional predictions, the candidate genes Zm00001eb023400, Zm00001eb023420 and Zm00001eb050490, which encoded lytic transglycosylases, significantly associated with cell wall remodeling and degradation. The candidate genes Zm00001eb282410 and Zm00001eb282430 enriched the phenylpropanoid biosynthesis pathway and played important roles in the formation of the maize ear shank. These findings provided a foundation for understanding the molecular mechanisms regulating ear shank length in maize.

キーワード:

BSA-seq 候補遺伝子穂柄長トウモロコシトランスクリプトーム解析

さらに関連する動画

10:08

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

10.1K

07:26

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

Published on: July 29, 2019

6.5K

関連する概念動画

01:19

X-linked Traits

In most mammalian species, females have two X sex chromosomes and males have an X and Y. As a result, mutations on the X chromosome in females may be masked by the presence of a normal allele on the second X. In contrast, a mutation on the X chromosome in males more often causes observable biological defects, as there is no normal X to compensate. Trait variations arising from mutations on the X chromosome are called “X-linked”.

01:43

Incomplete Dominance

Gregor Mendel's work (1822 - 1884) was primarily focused on pea plants. Through his initial experiments, he determined that every gene in a diploid cell has two variants called alleles inherited from each parent. He suggested that amongst these two alleles, one allele is dominant in character and the other recessive. The combination of alleles determines the phenotype of a gene in an organism.

01:19

X-linked Traits