関連する実験動画

Updated: Feb 26, 2026

Massively Parallel Reporter Assays in Cultured Mammalian Cells
11:03

Massively Parallel Reporter Assays in Cultured Mammalian Cells

Published on: August 17, 2014

22.5K

遺伝子特異的調節領域を単一ヌクレオチド分解能でマッピングするための大規模並列レポーターアッセイ

Alastair J Tulloch1,2, Ryan Nicholas Delgado1,2, Rinaldo Catta-Preta1,2

  • 1Department of Genetics, Harvard Medical School, Boston, United States.

eLife
|February 25, 2026
PubMed
まとめ

新しい方法である、遺伝子座特異的マッシブパラレルレポーターアッセイ（LS-MPRA）およびデジェネレートMPRA（d-MPRA）は、特定の細胞タイプにおけるシス調節モジュール（CRM）を効率的にマッピングします。これらのスケーラブルなツールは、種や組織を横断した遺伝子制御要素の発見を加速します。

キーワード:
Olig2ニワトリ染色体シス調節モジュール発生生物学遺伝子発現遺伝子調節マッシブパラレルレポーターアッセイマウス網膜前駆細胞

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Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq06:24

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関連する概念動画

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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