バーコード統合ヘリンボーンマイクロ流体デバイスによる膀胱がんエクソソームバイオマーカーのハイスループットスクリーニング

Bladder cancer (BC) ranks among the most prevalent urological malignancieswith high mortality, driving an urgent need for efficient, non-invasive diagnostic methods. In this work, we developed an integrated microfluidic platform that combines herringbone mixers with photonic crystal (PhC)-encoded, core-shell hydrogel microcarriers for efficient exosome enrichment and multiplexed biomarker detection. The core-shell hydrogel microcarriersoffer stable structural color barcoding immune to assay conditions and a substantiallyexpanded surface area for enhanced sensitivity. Concurrently, the herringbone structures generate controlled microturbulence, significantly improving fluid mixing and extending target-probe contact time. By employing this platform to profile a panel of six proteomic biomarkers derived from bladder cancer exosomes, we demonstrate efficient high-throughput analysisof urinary exosomal signatures. The platform thus shows excellent diagnostic performance, offering a promising approach for the early detection and monitoring of BC in clinical practice.