体外受精周期における着床前遺伝子検査（次世代シーケンサー使用）の有無別、選択的単一胚盤葉移植の無作為化臨床試験

関連する実験動画

Updated: Feb 28, 2026

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Published on: August 17, 2022

Pedro Felipe Magalhães Peregrino¹, Carlos Augusto Z Nissel¹, Patricia Florido¹

¹Disciplina de Ginecologia, Departamento de Obstetrícia e Ginecologia, Hospital das Clínicas da Faculdade de Medicina da Faculdade de Medicina da Universidade de São Paulo, São Paulo, SP, Brazil.

Clinics (Sao Paulo, Brazil)

|February 25, 2026

要旨

Introduction:

Preimplantation Genetic Testing (PGT-A) has been increasingly used in IVF to improve embryo selection. However, its clinical utility in patients with a good prognosis remains debated. There is a lack of robust scientific evidence about the benefits of preimplantation genetic testing Next-Generation Sequencing (NGS) on the clinical pregnancy and live birth rates in good-prognosis IVF patients undergoing elective Single Embryo Transfer (eSET) testing.

Objective:

To compare the clinical pregnancy rate after IVF treatment in patients with a good prognosis between groups that receive the transfer of a single embryo with or without pre-implantation genetic embryo analysis.

Methods:

This work was a prospective randomized clinical study that evaluated 206 patients to compare the clinical pregnancy rate after the first elective transfer of an embryo, associated or not with pre-implantation genetic embryo analysis using Next-Generation Sequencing (NGS). Patients were randomized into groups: SET group ‒ patients undergoing the transfer of a single embryo (eSET, elective Single Embryo Transfer) without embryo genetic analysis (SET group, n = 103); and NGS + SET group ‒ patients undergoing eSET with embryo genetic analysis (NGS + SET group, n = 103).

Results:

The clinical pregnancy rate after the first transfer was similar between the two groups (SET: 76.7 % and NGS + SET: 79.6 %; p = 0.613). There was also no statistical difference in live birth rates or abortion rates between the groups analyzed.

Conclusion:

This clinical trial suggests no significant improvement in the embryo genetic analysis in IVF treatments for infertile couples with a good prognosis.

キーワード:

体外受精次世代シーケンサー着床前遺伝子診断生殖医療単一胚盤葉移植妊娠率

さらに関連する動画

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Published on: August 25, 2019

Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis

Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis

Published on: September 7, 2021

関連する概念動画

In Vitro Fertilization

In Vitro Fertilization

In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....