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Updated: Feb 28, 2026

Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs

Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs

Published on: December 27, 2016

イグラチモドはアラキドン酸蓄積を介して体積感受性外向き整流性陰イオンチャネルを抑制する

Takahiro Shimizu¹, Shiho Sumiyoshi¹, Kyosuke Fujita²

¹Department of Pharmaceutical Physiology, Faculty of Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan.

European journal of pharmacology

|February 25, 2026

Abstract:

Iguratimod, an anti-rheumatic agent, is known to suppress the production of inflammatory cytokines and inhibit B-cell proliferation. Recent studies have implicated volume-sensitive outwardly rectifying (VSOR) Cl^- channels in the regulation of immune responses. In this study, we investigated whether iguratimod modulates VSOR Cl^- currents using whole-cell patch-clamp recordings in human embryonic kidney 293T cells. Iguratimod inhibited hypotonicity-induced VSOR Cl^- currents in a concentration-dependent manner. Notably, celecoxib, a selective cyclooxygenase-2 (COX-2) inhibitor, also significantly suppressed these currents. Furthermore, pyrrophenone, a phospholipase A₂ inhibitor, attenuated the inhibitory effect of iguratimod on VSOR Cl^- currents. Under hypotonic conditions, the levels of free arachidonic acid were significantly elevated in the presence of iguratimod and celecoxib. These findings suggest that iguratimod increases free arachidonic acid levels by inhibiting COX-2 during osmotic cell swelling, thereby suppressing VSOR Cl^- channel activity. This suppression of VSOR Cl^- channel activity may present one of the mechanisms through which iguratimod exerts its anti-inflammatory effects in rheumatic diseases.

キーワード:

アラキドン酸塩化物チャネルシクロオキシゲナーゼ-2 イグラチモド

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