原発性緬羊単球のロバストな分離による宿主細胞特異的感染研究

Benjamin U Bauer¹, Bidiepta Saha¹, Thierry Cottineau¹

¹Institute of Immunology, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald, Insel Riems, Germany.

|February 25, 2026

Abstract:

No widely available ovine monocyte/macrophage cell system exists. We present an affinity magnetic separation method combining gradient separation and magnetic bead selection to isolate pure primary ovine monocytes from blood, yielding large quantities of viable primary macrophages suitable for microbiological infection studies using reservoir-specific host cells targeted by intracellular pathogens.

キーワード:

クラミジア単球由来マクロファージ PBMC 羊人獣共通感染症コクシエラ

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Overview Of Cell Separation And Isolation

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