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Transgenic Plants02:50

Transgenic Plants

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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
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Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

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Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
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Protein Transport to the Outer Chloroplast Membrane01:11

Protein Transport to the Outer Chloroplast Membrane

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Chloroplast outer membrane proteins encoded by the nucleus are synthesized in the cytosol. Soon after synthesis, they bind cytosolic factors such as 14-3-3 protein and the Hsp70 chaperones that keep these precursors in an unfolded state until their translocation.
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.
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Protein Transport to the Inner Chloroplast Membrane01:18

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Proteins targeted to the inner chloroplast membrane, or plastid proteins, are transported by two general pathways: the stop-transfer and the re-insertion or post-import pathways. Most plastid proteins carry N-terminal transit sequences and internal import sequences targeting it to the specific chloroplast subcompartment. Proteins targeted by the stop-transfer pathway have internal hydrophobic sequences that inhibit their translocation into the stroma. As a result, these precursors are arrested...
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Overview of Protein Sorting and Transport01:45

Overview of Protein Sorting and Transport

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Eukaryotic cells have different membrane-bound organelles with distinct protein requirements. The process by which proteins are targeted to a specific organelle is called protein sorting.
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Transgenic Organisms00:53

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関連する実験動画

Updated: Feb 28, 2026

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants
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植物への機能性タンパク質のシステム的送達のためのエンジニアリング膜移行ドメインの使用

Jiyang Wang1, Preeti Patel1, Prabhat Bhat2

  • 1Department of Plant Pathology, Ohio State University, Columbus, Ohio, USA.

Plant biotechnology journal
|February 26, 2026
PubMed
まとめ

新しいエンジニアリングされたタンパク質送達システムであるMTD4は、植物へのバイオ農薬の効果的なシステム的取り込みを可能にします。この技術は、持続可能な農業のための化学薬品の使用への依存を減らし、病気に対する作物保護を大幅に強化します。

キーワード:
細胞浸透防御活性化因子膜移行タンパク質送達持続可能な農業

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関連する実験動画

Last Updated: Feb 28, 2026

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科学分野:

  • 植物バイオテクノロジー; 農業科学; 分子生物学

背景:

  • タンパク質ベースのバイオ農薬およびバイオスティミュラントは、持続可能な農業に不可欠です。現在の植物細胞へのタンパク質送達方法は非効率的であり、作物生産における全身活性が欠如しています。

研究 の 目的:

  • 頑丈でスケーラブルなタンパク質を作物に送達するための新しいエンジニアリング膜移行ドメイン（MTD4）を開発すること。MTD4が植物全体にわたる全身タンパク質移行を促進する能力を実証すること。

主な方法:

  • 効率的な葉面および根からシュートへのタンパク質送達のためにMTD4をエンジニアリングしました。植物防御応答の誘発における有効性をテストするために、MTD4をハープタンパク質HrpZ（MTD4-HrpZ）に融合しました。タバコおよびトマト植物にMTD4-HrpZを適用して、病気の軽減を評価しました。

主要な成果:

  • MTD4は、葉や根からのモデルタンパク質（SEP）の迅速な葉面送達と全身移行を可能にしました。MTD4-HrpZは、植物において強力な過敏反応と全身獲得抵抗性を誘導しました。細菌および真菌の病気の重症度は大幅に減少し、MTD4-HrpZはHrpZ単独よりも5倍以上効果的でした。

結論:

  • MTD4は、植物におけるタンパク質送達の障壁を克服するための革新的なツールです。このプラットフォームは、高度な持続可能な作物保護のための新しい世代の高効力バイオセラピューティクスを可能にします。MTD4技術は、農業における化学農薬への依存を減らすことができます。