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Updated: Mar 3, 2026

Author Spotlight: Enhancing Multicolor Fluorescence Localization in Lung Carcinoma Sample

Author Spotlight: Enhancing Multicolor Fluorescence Localization in Lung Carcinoma Sample

Published on: November 21, 2023

肺扁平上皮癌におけるAIREの核発現は免疫微小環境の再構築と予後不良に関連する

Chengyan Wang^1,2, Chuhuai Wang³, Yuhan Jia¹

¹Department of Oncology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

|March 2, 2026

要旨

Objective:

The autoimmune regulator (AIRE), an atypical transcriptional regulator, exhibits aberrant expression in multiple tumor types. However, its significance in lung squamous cell carcinoma (LUSC) is still uncertain. This study investigated AIRE's expression, immune microenvironment association, and prognostic value in LUSC.

Method:

AIRE expression was analyzed using TCGA database, validated via RT-qPCR/Western blot in LUSC (H1703, SK-MES-1, EBC-1) vs. normal (BEAS-2B) cells. Tissue microarray immunohistochemistry (IHC) quantified nuclear/cytoplasmic AIRE in clinical samples. Clinicopathological correlations, immune infiltration (CIBERSORT), and survival outcomes were assessed. A nomogram integrating nuclear AIRE and clinical factors predicted 1/3/5-year survival (C-index evaluation).

Result:

AIRE was upregulated in LUSC. Nuclear AIRE was tumor-specific (absent in normal tissue), elevated in inflammatory stroma (6.29 ± 4.57 vs. 3.10 ± 3.66, P < 0.001), and correlated with lymphocyte infiltration (R = 0.437, P < 0.001). High AIRE linked to increased Tregs (P< 0.001) and reduced dendritic cells (P< 0.01). No association with age, stage, or Ki-67 (P > 0.05) was observed. Patients with high AIRE had worse survival (HR = 2.00 [1.02 - 3.93], P = 0.043). The nomogram incorporating nuclear AIRE achieved a C-index of 0.814 for outcome estimation in our cohort.

Conclusion:

In a LUSC cohort, nuclear AIRE expression is associated with immune microenvironment features and poorer overall survival. However, its incremental prognostic value beyond established clinicopathological variables appears modest, and further external validation and functional studies are warranted.

キーワード:

AIRE 肺扁平上皮癌予後腫瘍微小環境

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Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Published on: September 20, 2024

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

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