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Updated: May 9, 2026

Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants
08:26

Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants

Published on: August 31, 2016

グロービンとコリオンmRNAの全長および離散部分逆転写

A Efstratiadis, T Maniatis, F C Kafatos

    Cell
    |April 1, 1975
    PubMed
    まとめ

    研究者はAMV逆転写酵素を使用して,ウサギのグロービンmRNAから補完的なDNA (cDNA) を合成しました. デオキシリボヌクレオチド (dNTP) の濃度を最適化すると,全長cDNAの生成が著しく増加し,完全なグロービンmRNAトランスクリプトが得られます.

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