膀性ストマチスウイルスは,ポラライズされた上皮細胞系,MDCKKの基礎側表面を通してのみ感染し,成熟します
Cell
|August 1, 1984
まとめ
Madin-Darby犬の腎臓細胞は,独特のウイルス感染と芽生えた極性を示しています. 膀性口炎ウイルス (VSV) は,好ましくは下側から感染し,芽を芽生えますが,鳥の疫病ウイルス (FPV) は,より低い極性を示します.
科学分野:
- 細胞生物学 細胞生物学
- ウイルス学 ウイルス学 ウイルス学
- エピテル細胞生物学 エピテル細胞生物学
背景:
- 偏極化された上皮細胞は,独特の頂上および底側膜領域を示します.
- ポラライズされた細胞にウイルスの侵入と集合を理解することは,ウイルスの病原化と制御に不可欠です.
研究 の 目的:
- Madin-Darby canine kidney (MDCK) 細胞単層で,小胞性口腔炎ウイルス (VSV) と鳥インフルエンザウイルス (FPV) の感染と成熟の極性を調べる.
- ポラライズされた表皮のモデルで,ウイルスの侵入と芽生えの好ましい場所を決定する.
主な方法:
- ナイトロセルロースフィルターで育ったマディン・ダービー犬腎臓 (MDCK) 細胞を用いて,極化した表皮単層を形成した.
- VSVとFPVをアピカルとベソラテラル表面に適用することで,ウイルス感染の効率を評価した.
- 細胞単層の両側からウイルスの放出を測定することによって,ウイルスの芽の極性を定量化しました.
主要な成果:
- MDCK細胞は,高度に耐性のある上皮単層を形成した.
- VSVは,アピカル表面よりも約100倍効率的にベースラテラル表面に感染しました.
- 芽生えたVSVの98%以上が基礎側から放出され,FPVの99%以上のヘマグルチニンはアピカルに放出されました.
- 芽生えと表面タンパク質発現の2極性は少なくとも10時間維持された.
結論:
- VSVは感染と成熟の両方において強い極性を示し,好ましくは基礎側膜を利用する.
- FPVは,感染の際の極性性がはっきりしないが,芽は主にアピカルである.
- MDCK細胞単層は,ウイルス-表皮の相互作用と極性を研究するための貴重なモデルとして機能します.
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