関連する実験動画
Updated: May 12, 2026

16:47
Dissection of a Mouse Eye for a Whole Mount of the Retinal Pigment Epithelium
Published on: February 28, 2011
ネズミのコラーゲンI遺伝子におけるレトロウイルス誘発の致死性突然変異は,染色体構造の変化と関連しています
Cell
|August 1, 1984
まとめ
Mov13マウスにレトロウイルスを挿入すると,コラーゲン遺伝子の染色体構造が破壊されます. これは,重要な転写関連過敏部位を阻害し,胚の発達中の遺伝子活性化を阻害します.
科学分野:
- 分子生物学は分子生物学である.
- 発達生物学 発達生物学について
- 遺伝学 遺伝学とは
背景:
- コラーゲンα1 (I) 遺伝子は,胚の発達に不可欠です.
- Mov13マウスモデルは,この遺伝子のレトロウイルス挿入変異を携えています.
- クロマチンの変化を理解することは,遺伝子調節障害の解読の鍵です.
研究 の 目的:
- ワイルドタイプコラーゲンアルファ1 (I) 遺伝子の染色体構造を,Mov13マウスにおけるレトロウイルス変異アレルと比較する.
- 正常アレルと突然変異アレル間のDNAase I過敏部位の違いを特定する.
- レトロウイルスの挿入が発達中の遺伝子活性化に影響を与えるメカニズムを解明する.
主な方法:
- クロマチンのアクセシビリティを検知するために,限られたDNAase I消化を利用した.
- ワイルドタイプとMov13マウスの様々な細胞タイプにおけるクロマチンの構造を分析した.
- コラーゲンアルファ1 (((I)) mRNA合成と過敏部位の存在/欠如を相関させた.
主要な成果:
- ワイルドタイプおよび突然変異のコラーゲンα1 (I) 遺伝子クロマチンの2つの安定したDNAase I過敏部位を特定しました.
- 発端部位に対する第3の転写関連過敏部位5'が発見され,コラーゲンα1 (I) mRNAを積極的に合成する細胞のみに存在する.
- この転写関連部位は変異したMov13アレルクロマチンに存在しないが,他の2部位はプロウイルス統合の影響を受けなかった.
結論:
- Mov13マウスのレトロウイルスの挿入は,コラーゲンα1 (I) 遺伝子の活性化に必要な正常な染色体改造を妨げます.
- ウイルスの挿入は,転写関連過敏部位の発達的に調節された外観に干渉する可能性が高い.
- このクロマチンの変異は,Mov13マウスで観察された胚の発達障害の分子説明を提供します.
関連する概念動画
In-vitro Mutagenesis
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Conservative Site-specific Recombination and Phase Variation
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
Lethal Alleles
Agouti: A Lethal Allele
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...
Mouse Models of Cancer Study
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
Type IV Collagen of Basal Lamina
Type IV collagen is a 400 nm long, network-forming collagen that acts as a barrier between the epithelial and endothelial cells. Type IV collagen forms the backbone of the basement membrane by scaffolding with laminin, entactin, proteoglycans, and fibronectin. Apart from rendering structural support to the basement membrane, it also helps entail signaling potentials necessary for both pathological and physiological functions.
A type IV collagen molecule has six alpha chains which can exist in...
A type IV collagen molecule has six alpha chains which can exist in...

