ヒトのトランスファーリン受容体遺伝子:ゲノム組織と,cDNA配列から推論された受容体の完全な主要な構造
Cell
|December 1, 1984
まとめ
転送リン受容体遺伝子は19のコード配列を持っています. その推論されたアミノ酸配列は,細胞質のN末端と細胞外C末端を持つトランスメブラン的指向を示唆する.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- 細胞生物学 細胞生物学
背景:
- トランスファーリン受容体は,細胞の鉄吸収に不可欠です.
- その遺伝子構造とタンパク質配列を理解することは,その機能を明らかにする鍵です.
研究 の 目的:
- 転送リン受容体遺伝子のコーディング領域の核酸配列を決定する.
- 転送リン受容体タンパク質の構造と膜方向を予測する.
主な方法:
- 転送リン受容体遺伝子のヘテロデュプレックス解析.
- cDNAクローンのヌクレオチド配列決定.
- 推論されたアミノ酸配列解析.
主要な成果:
- ゲノムDNAの31kBをカバーする少なくとも19のコーディングシーケンスが特定されました.
- 2280塩基の完全な開いた読み取りフレームを決定し,760残留,85 kDaのポリペプチドを指定しました.
- シングルトランスメブランドメインを予測し,シトプラズマのN端と細胞外C端を示した.
- トランスファーリンまたは他の既知の受容体との有意なホモロジーは見つかりませんでした.
結論:
- トランスファーリン受容体遺伝子は,広範にコード化されています.
- 推論されたタンパク質配列と予測された膜トポロジーは,受容体の機能と局所化に関する洞察を提供します.
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