人間の骨肉肉腫細胞系におけるPDGFのような成長因子とPDGF受容体の共同発現:オトクリン受容体活性化への影響
Cell
|December 1, 1984
まとめ
人間の骨髄腫細胞はPDGFのような成長因子と受容体を発現し,構成経路の活性化につながります. しかし,このオトクリン信号は,腫瘍の成長や変容を誘導しない可能性があります.
科学分野:
- 細胞生物学 細胞生物学
- 分子腫瘍学 分子腫瘍学
- シグナルトランスデュークション
背景:
- オステオサルコマは悪性骨腫瘍である.
- 血小板由来成長因子 (PDGF) のシグナル伝達は,正常な細胞成長において極めて重要であり,がんでは失調することがあります.
研究 の 目的:
- ヒトの骨肉肉腫細胞系 (U-2 OS Cl 6) でのPDGFのような成長因子とその受容体の発現と機能を調査する.
- PDGF受容体依存経路がこれらの細胞で構成的に活性化されているかどうかを決定し,変換におけるその役割.
主な方法:
- U-2 OS Cl 6細胞におけるPDGFのような成長因子と機能的なPDGF受容体発現の実証.
- これらの分子の相互作用によるチロシン酸化とアクチン再構成の分析.
- 構成的にリン酸化されたタンパク質を識別するための免疫プレシピテーション.
- 細胞外PDGF抗体の細胞成長および変異した性質に対する影響の評価.
主要な成果:
- U-2 OS Cl 6細胞はPDGFのような成長因子と機能的なPDGF受容体を発現し,チロシンリン酸化とアクチン再構成を媒介する.
- 115 kDaのタンパク質は,PDGFで刺激された正常な線維芽細胞とは異なり,U-2 OS Cl 6細胞で構成的にリン酸化されました.
- PDGF受容体に依存する経路は,この骨髄腫細胞系において構成的に活性化されています.
- 細胞外PDGF抗体は,U-2 OS Cl 6細胞の変形した性質や成長率を変化させなかった.
結論:
- PDGF受容体依存シグナル伝達経路は,U-2 OS Cl 6骨肉腫細胞系で構成的に活性化されています.
- オトクリンPDGF受容体の活性化は,この特定の腫瘍細胞系の変異状態または成長率を維持するために重要ではないようです.
- セル外抗体にはアクセスできない,オトクリンシグナル伝達のための代替的な細胞内コンパートメントが存在することがあります.
さらに関連する動画
関連する概念動画
Mitogens and the Cell Cycle
6.4K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.4K
Abnormal Proliferation
4.0K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.0K
The Ras Gene
5.7K
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
Ras is a...
5.7K
Regulation of Angiogenesis and Blood Supply
2.9K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.9K
Amplifying Signals via Enzymatic Cascade
15.3K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
15.3K
TGF - β Signaling Pathway
7.2K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.2K


