スクイードの巨大アクソンから挤出されたアクソプラズムの急速なアクソナル輸送
まとめ
スクイードの巨大アクソンの膜性臓器細胞の急速な軸索輸送は,プラズマ膜なしで続きます. この発見は,プラズマ膜とアクションポテンシャルが,急速な軸索輸送を調節するために不可欠ではないことを示しています.
科学分野:
- 神経科学は神経科学である.
- 細胞生物学 細胞生物学
背景:
- 急速な軸索輸送は,軸索内の臓器細胞の移動を含む神経機能に不可欠です.
- 以前の研究は,無傷の軸索性プラズマ膜の必要性によって制限されていました.
研究 の 目的:
- 急速な軸索輸送を調節するプラズマ膜の役割を調査する.
- 膜の障壁から独立して軸索輸送を研究するためのモデルシステムを開発する.
主な方法:
- ビデオ強化コントラスト差異干渉コントラスト顕微鏡を用いた.
- プラズマ膜が欠けているイカの巨大アクソンから抽出されたアクソプラズマを研究した.
主要な成果:
- 膜性臓器細胞 (30 nm の膀から 5000 nm のミトコンドリアまで) は正常なオーソグラードと逆行移動を示した.
- 素早い軸索輸送は,細胞フリーのアクソプラズマ製剤で数時間持続しました.
結論:
- 急速な軸索移動には,軸索性プラズマ膜は必要ありません.
- アクションポテンシャルは,急速な軸索輸送を調節する可能性は低い.
- 細胞のないアクソプラズマは,有機細胞輸送の生化学的および薬理学的研究のための貴重なモデルを提供します.
関連する概念動画
Action Potentials
Overview
Microtubules in Cell Motility
Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
Action Potential
Neurons communicate by firing action potentials—the electrochemical signal that is propagated along the axon. The signal results in the release of neurotransmitters at axon terminals, thereby transmitting information to the nervous system. An action potential is a specific "all-or-none" change in membrane potential that results in a rapid spike in voltage.
Membrane potential in neurons
Neurons typically have a resting membrane potential of about -70 millivolts (mV). When they receive...
Membrane potential in neurons
Neurons typically have a resting membrane potential of about -70 millivolts (mV). When they receive...
Neurons: The Axon
Axons are long, cytoplasmic processes of nerve cells capable of propagating electrical impulses known as action potentials. The cytoplasm or axoplasm of an axon contains neurofibrils, neurotubules, small vesicles, lysosomes, mitochondria, and various enzymes, all encased within the axolemma, the plasma membrane of the axon.
The axon attaches to the cell body at a cone-shaped elevation called the axon hillock. The initial part of the axon, closest to the hillock, is known as the initial segment.
The axon attaches to the cell body at a cone-shaped elevation called the axon hillock. The initial part of the axon, closest to the hillock, is known as the initial segment.
Action Potential
Neurons communicate by firing action potentials—the electrochemical signal that is propagated along the axon. The signal results in the release of neurotransmitters at axon terminals, thereby transmitting information to the nervous system. An action potential is a specific "all-or-none" change in membrane potential that results in a rapid spike in voltage.
Membrane potential in neurons
Neurons typically have a resting membrane potential of about -70 millivolts (mV). When they receive...
Membrane potential in neurons
Neurons typically have a resting membrane potential of about -70 millivolts (mV). When they receive...
Propagation of Action Potentials
The propagation of an action potential refers to the process by which a nerve impulse, or "action potential," travels along a neuron.
Neurons (nerve cells) have a resting membrane potential, with a slightly negative charge inside compared to outside. This is maintained by ion channels, such as sodium (Na+) and potassium (K+) channels, which control the flow of ions. When a stimulus, like a touch or a signal from another neuron, triggers the neuron, sodium channels open, allowing sodium ions to...
Neurons (nerve cells) have a resting membrane potential, with a slightly negative charge inside compared to outside. This is maintained by ion channels, such as sodium (Na+) and potassium (K+) channels, which control the flow of ions. When a stimulus, like a touch or a signal from another neuron, triggers the neuron, sodium channels open, allowing sodium ions to...


