U4のような小さな核RNAは,酵母に欠かせないものです

D Tollervey, J A Wise, C Guthrie

Cell

|December 1, 1983

まとめ

研究者らは,酵母におけるsnR3遺伝子の機能を調査した. 驚くべきことに,snR3遺伝子を欠いた酵母細胞は生存可能であり,成長欠陥を示せず,以前の仮定に異議を唱えた.

科学分野:

分子生物学は分子生物学である.
遺伝学遺伝学とは
イースト生物学イースト生物学

背景:

小型核RNA (snRNA) は,細胞の様々なプロセスに不可欠です.
イーストのsnR3遺伝子は,哺乳類のU4 snRNAとの配列と構造的な類似性を持つ小さな核RNAをコードする.
Saccharomyces cerevisiaeにおけるsnR3の本質性は,以前は未定であった.

研究の目的:

イーストのsnR3遺伝子の本質を調査する.
Saccharomyces cerevisiaeにおけるsnR3の機能的役割を決定する.

主な方法:

Saccharomyces cerevisiaeから単一コピーのsnR3遺伝子をクローン化しました.
コード配列をLEU2遺伝子に置き換えることでSNR3の遺伝子破壊.
機能的なSNR3が欠けているハプロイド株の分析は,DNAブラットハイブリダイゼーションと成長アッセイを介して行われます.

主要な成果:

SNR3遺伝子の破壊は,生命不能な細胞を生じさせなかった.
検出可能なsnR3トランスクリプトが欠けているハプロイド酵母菌株は生存可能であった.
これらのsnR3欠乏菌株は,テストされた成長条件下では,野生型の菌株と相違しない現象型であった.

結論:

snR3遺伝子は,標準的な実験室条件下での酵母生存または成長に不可欠ではありません.
酵母におけるsnR3の機能は,余剰または生存に欠かせない可能性があります.
Saccharomyces cerevisiaeにおけるsnR3の正確な役割を明らかにするために,さらなる研究が必要である.

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