大型リボソーム前駆体RNAのスプライシングと酵母ミトコンドリアにおけるイントロンRNAの処理
Cell
|December 1, 1984
まとめ
イーストミトコンドリアRNAスプライシングは,線形RNAや円形RNAのような中間物質を含みます. 切断されたイントロンは,循環させたり,内部の割れ目を経験したりすることができ,テトラヒメナの自己接合に似ています.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- バイオケミストリー バイオケミストリー
背景:
- イーストにおけるミトコンドリアの遺伝子発現は,複雑なRNA処理を伴う.
- リボソームRNA (rRNA) の合成と成熟は,細胞の機能に極めて重要です.
研究 の 目的:
- イーストミトコンドリアの大型リボソームRNA前駆体のスライシングメカニズムを調査する.
- 切断されたイントロンの処理経路を特徴付けるために.
主な方法:
- リバーストランスクリプタゼによるプライマー拡張.
- 電子顕微鏡でRNAの構造を視覚化します.
主要な成果:
- 特定された線形RNA中介物質は,コード化されていない5'-末端のGを持つ.
- イントロン境界の共振結合によって形成される円形のRNA中間物質について説明します.
- 5'イントロン境界の3つの核酸が循環形に欠けていることが観察されました.
- 切断されたイントロンが循環化したり,内部分裂したりすることを示した.
結論:
- 観察されたスプライシング中間物質と処理経路は,テトラヒメナ核rRNAに似た自己スプライシングメカニズムと一致しています.
- 酵母菌のミトコンドリアイントロンは,循環化または内部分裂という二重の処理運命を表しています.
関連する概念動画
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02:01Pre-mRNA Processing
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
01:32RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
01:35Pre-mRNA Processing: Modification of pre-mRNA Ends
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
01:32Pre-mRNA Processing: RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...