ミトスの染色体におけるDNAase I 敏感領域のマッピング

B S Kerem, R Goitein, G Diamond

Cell

|September 1, 1984

まとめ

非活性X染色体は,活性染色体よりもDNAase I消化に敏感性が低い. この染色体構成の差異は,in situ nick翻訳で検出され,ヒトとCHO染色体における異なる帯状パターンを明らかにします.

科学分野:

分子生物学は分子生物学である.
遺伝学遺伝学とは
クロマチンの構造クロマチンの構造

背景:

哺乳類の雌の不活性X染色体は,独特の染色体構成を示しています.
DNAase Iの感受性は,染色体内の活性遺伝子領域の既知の指標である.
以前の研究では,クロマチンの形状が固定されたミトーシス染色体で維持されていることが示唆されました.

研究の目的:

染色体分析のための in situ nick 翻訳技術を精錬し,検証する.
固定されたヒトとCHO染色体におけるDNAase I感受性のパターンを調査する.
染色体帯とヘテロクロマチン領域とのDNAase I感受性を相関させるため.

主な方法:

バイオチニル化dUTPと非放射性検出システムを使用して,インシットニック翻訳の強化.
精製されたメソッドをヒトおよびCHO細胞からの固定ミトーシス染色体に適用する.
特定の帯状パターンを特定するために,DNAアゼI感受性および無感受性領域の分析.

主要な成果:

DNAase I 敏感および不敏感の染色体領域は,独特の暗 (D-バンド) と明るい帯状のパターンを生成しました.
DNAase I 敏感な D-バンドは,一般的に光の G-バンドと一致しますが,普遍的ではありません.
不活性な構成ヘテロクロマチン領域は一貫してDNAase I無感性であった.

結論:

精巧な in situ nick 翻訳方法は,固定された染色体内の染色体の構造的組織を正確に視覚化します.
特定のDNAase I感受性のパターンは,クロマチンの機能状態を反映し,活性および非活性領域を区別します.
この技術は,染色体構造と機能を研究するための貴重な新しいツールを提供します.

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