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関連する概念動画

Chromosome Structure02:40

Chromosome Structure

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A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

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DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
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Gene Conversion02:08

Gene Conversion

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Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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Crossing Over

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Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I,...
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Updated: May 5, 2026

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
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Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

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ラムダ局所固有の再結合における方向性の決定因子

W Bushman, S Yin, L L Thio

    Cell
    |December 1, 1984
    PubMed
    まとめ
    この要約は機械生成です。

    DNAの構造的特徴が,ラムダ部位特異再結合における方向性を決定する. シスタンパク質 (Xis protein) とは

    さらに関連する動画

    Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
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    関連する実験動画

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    Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
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    科学分野:

    • 分子生物学は分子生物学である.
    • 遺伝学 遺伝学とは
    • バイオケミストリー バイオケミストリー

    背景:

    • ラムダ部位固有の再結合は,ウイルスのゲノム統合と切除のための重要なプロセスです.
    • この再結合の方向性は,ファグの結合部位内の特定のDNA配列によって影響を受けます.
    • 統合と切除を調節するXisタンパク質の役割は観察されていますが,分子レベルで完全に解明されていません.

    研究 の 目的:

    • ラムダサイト固有の再結合における方向性に関与するDNAの構造特性を特定し,特徴づけること.
    • シスタンパク質が統合反応と切除反応に差異的に影響するメカニズムを解明する.
    • シスタンパク質の結合特性と,Intタンパク質の結合に及ぼす影響を調査する.

    主な方法:

    • DNAフットプリント測定は,規制タンパク質の結合部位を特定するための測定である.
    • 付着部位内の特定のDNA配列を変更するためのサイト指向型変異.
    • タンパク質とDNAの相互作用を測定するためのゲル電泳と定量結合測定法.
    • 配列変化とタンパク質結合の影響を決定するために再結合製品の分析.

    主要な成果:

    • 再結合の方向性を支配するDNAの重要な構造的特徴は,クロスオーバー領域の左70pb,右40pb以上に位置しています.
    • これらの配列の空間的配置 (同じ部位または異なる部位) は,統合 (抑制) と切除 (刺激) でシスタンパク質の対極的な役割を決定する.
    • Xisタンパク質は,左のファグアームの隣接する直接のリピートに協力的に結合し,DNA構成の変化を誘導し,Intタンパク質の結合を32倍に高めます.

    結論:

    • この研究は,特定のDNA領域とその空間的配置を,ラムダ再結合における方向性の決定的決定因子として特定しています.
    • 統合と切除に対するXisタンパク質の異なる効果は,異なる配列配列との相互作用によって説明されます.
    • シスタンパク質は,協力的なDNA結合と形状の変化を通じてInt結合の強力な調節剤として作用し,それによって再結合方向性を制御します.