鳥肉腫ウイルス変異タンパク質,pp60srcはチロシンに特異的なタンパク質キナーゼ活性を示しています

M S Collett, A F Purchio, R L Erikson

Nature

|May 15, 1980

まとめ

鳥肉腫ウイルス (ASV) フォスフォタンパク質 pp60srcは,タンパク質キナーゼである. このタンパク質キナーゼは,様々な基板のチロシン残基をリン酸化し,悪性変異に重要な役割を果たします.

科学分野:

分子生物学は分子生物学である.
ウイルス学ウイルス学ウイルス学
バイオケミストリーバイオケミストリー

背景:

鳥肉腫ウイルス (ASV) は悪性変異を引き起こす.
ASVタンパク質 pp60srcは,この変換に関与しています.
pp60srcの細胞タンパク質のリン酸化における役割は,以前から示唆されていた.

研究の目的:

ASV変換タンパク質 pp60src.を生化学的に浄化し,特徴づけること.
pp60srcが酵素活性を持っているかどうかを判断します.
pp60src.srcによって媒介される特定のタイプのリン酸化を特定する.

主な方法:

イオン交換と免疫親和染色を用いたpp60srcの生化学的浄化.
精製されたpp60src製剤におけるタンパク質キナーゼ活性に関する測定.
基板タンパク質のリン酸化残留物の分析.

主要な成果:

pp60srcは,約5,000倍に精製されました.
浄化されたpp60srcは,周期的なAMP-独立のタンパク質キナーゼ活性を示す.
このキナーゼの活動は,特に様々なタンパク質基板のチロシン残基をリン酸化します.

結論:

ASV変換遺伝子の産物であるpp60srcは,タンパク質キナーゼである.
pp60srcはチロシン残基のリン酸化を媒介する.
このチロシンキナーゼの活動は,ASV誘発の悪性変異の中心にある可能性が高い.

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