関連する実験動画

Updated: Jul 28, 2026

Generating Chimeric Zebrafish Embryos by Transplantation
21:01

Generating Chimeric Zebrafish Embryos by Transplantation

Published on: July 17, 2009

鶏のチミジンキナーゼ遺伝子の分離は,プラズミドによる救出によるものです

M Perucho, D Hanahan, L Lipsich

    Nature
    |May 22, 1980
    PubMed
    まとめ

    研究者らは,細菌のプラズミドpBR322.2を用いてチキンチミジンキナーゼ (tk) 遺伝子をクローンした. この遺伝子はtk-動物細胞を効果的に変容させ,ウイルス tk遺伝子の効率に匹敵します.

    さらに関連する動画

    CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models10:57

    CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

    Published on: August 24, 2022

    Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish09:17

    Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

    Published on: July 22, 2025

    関連する概念動画

    In-vitro Mutagenesis01:16

    In-vitro Mutagenesis

    To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
    Position-effect Variegation02:32

    Position-effect Variegation

    In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
    Animal Mitochondrial Genetics02:59

    Animal Mitochondrial Genetics

    Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
    Gene Conversion02:08

    Gene Conversion

    Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
    Genomic Imprinting and Inheritance02:30

    Genomic Imprinting and Inheritance

    Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
    The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
    Chromatin Immunoprecipitation- ChIP02:36

    Chromatin Immunoprecipitation- ChIP

    Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
    Types of ChIP
    ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...