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関連する概念動画

Nucleotide Excision Repair01:08

Nucleotide Excision Repair

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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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Restriction Enzymes01:11

Restriction Enzymes

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Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
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Nucleotide Excision Repair01:38

Nucleotide Excision Repair

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DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
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Mismatch Repair01:20

Mismatch Repair

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Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
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Inducible Operons: lac Operon01:25

Inducible Operons: lac Operon

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The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA...
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The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker
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ラムダ部位特異再結合に必要なE. coliの遺伝子産物.

H I Miller, D I Friedman

    Cell
    |July 1, 1980
    PubMed
    まとめ
    この要約は機械生成です。

    E. coli の himA 遺伝子の変異は,バクテリオファージのラムダリンソゲニーに不可欠なサイト特異的再結合を阻害する. himAタンパク質は,DNAとタンパク質の相互作用を促進し,様々なファグの統合と切除プロセスに影響を与える可能性があります.

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    Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
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    Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
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    The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker
    12:03

    The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker

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    Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
    11:40

    Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

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    Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
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    科学分野:

    • 分子生物学は分子生物学である.
    • 遺伝学 遺伝学とは
    • 微生物学 微生物学とは

    背景:

    • サイト固有の再結合は,バクテリオファージのラムダリンソゲニーにとって極めて重要です.
    • この過程における himA 遺伝子の役割は,以前は不明でした.
    • 彼の機能を理解することは,DNA再結合メカニズムを解読する鍵です.

    研究 の 目的:

    • 彼を特徴付けるには,E. coli. の突然変異がありました.
    • サイト固有の再結合における himA遺伝子産物の機能を明らかにする.
    • ヒムA変異のプレオトロプ的効果を調査するために.

    主な方法:

    • ラムダサイト固有の再結合をサポートできないE. coli himA変異体の選択.
    • 彼のA変異の遺伝子マッピング.
    • コンプリメンテーションテストと優位性研究.
    • 様々なファグの統合および切除のイベントに対するヒムA変異の効果の分析.

    主要な成果:

    • 3つの非補完のヒマ変異が特定され,1つは無意味な変異であり,タンパク質製品であることを確認しました.
    • himA変異は,バクテリオファージ・ラムダの統合性および分離性サイト固有の再結合を著しく減少させた.
    • himAの突然変異は,他のファグの統合,ファグ muの成長,ファグ muとTn要素の正確な切除に影響を与えるプレオトロピック効果を示した.

    結論:

    • himAタンパク質は,サイト固有の再結合に不可欠であるか,またはそれを調節する可能性がある.
    • himA変異のプレオトロプ的性質は,タンパク質とDNAの相互作用を促進する一般的な役割を示唆しています.
    • him タンパク質は,さまざまなDNA操作プロセスにおいて補助因子として作用する可能性があります.