交配信号は,異なった酵母遺伝子の隣接する転置可能な繰り返し要素の挿入によって生じる変異の発現を制御する
Cell
|November 1, 1980
まとめ
イーストのROAM変異は,特にハプロイド系株において,遺伝子の過剰生産を引き起こします. この過剰生産は結合能力と関連しており,遺伝子の近くにTy要素の挿入が含まれています.
科学分野:
- 分子生物学は分子生物学である.
- イースト遺伝学 イースト遺伝学
背景:
- Saccharomyces cerevisiaeにおけるROAM変異は,遺伝子産物の過剰生産につながる.
- 過剰生産の程度は,異なる酵母交配タイプとプロイディレベルによって異なります.
研究 の 目的:
- 酵母におけるROAM変異の基礎となる遺伝的基礎と規制メカニズムを調査する.
- ROAM媒介による遺伝子発現におけるTy要素の役割を決定する.
主な方法:
- Saccharomyces cerevisiae.におけるROAM変異体の遺伝子解析について
- ROAM変異遺伝子CYC7-H2.2.のクローニングと特徴づけ
- 結合に影響する突然変異 (ste突然変異) がROAM発現に与える影響を調査する.
主要な成果:
- 過剰生産は,結合する能力のあるハプロイド株で,結合しない二プロイドと比較して高くなっています.
- 交配できる二倍性菌株は,胞子化能力に関係なく,過剰生産を示します.
- CYC7の5'ノンコーディング領域にTy1要素を挿入することは,ROAM変異体で確認されています.
- 結合を防ぐste7変異は,ROAM過剰生産を減少させます.
結論:
- ROAM変異は,Ty要素の挿入と関連しており,遺伝子発現が変化した可能性が高い.
- ROAM変異は,酵母結合を調節する信号に反応するようです.
- Ty要素配列は,結合に関連する規制信号の受容体として作用する可能性があります.
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