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Updated: Jul 10, 2026

Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo
10:51

Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo

Published on: October 31, 2014

クセノプス5SのDNAの核酸配列は,転写終了に必要なものです

D F Bogenhagen, D D Brown

    Cell
    |April 1, 1981
    PubMed
    まとめ

    Xenopus 5SのRNA合成の終了には,DNAにT残基のクラスターが必要です. 開始と終了のためのシーケンス信号は分離可能であり,独立して機能し,GCが豊富な領域が効率性を高めます.

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    Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq10:23

    Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

    Published on: February 26, 2015

    Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach12:12

    Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach

    Published on: March 12, 2017

    関連する概念動画

    Eukaryotic RNA Polymerases00:58

    Eukaryotic RNA Polymerases

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    All three eukaryotic RNAPs require specific transcription factors, of which the...
    Transcription Elongation Factors02:35

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    The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
    Transcription Attenuation in Prokaryotes02:42

    Transcription Attenuation in Prokaryotes

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    Bacterial Transcription01:53

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    Transcription Initiation01:47

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    Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
    The promoters and enhancers and their accessory proteins allow tight regulation of...
    Transcription in Prokaryotes01:28

    Transcription in Prokaryotes

    Transcription is a highly regulated process that converts genetic information into RNA molecules. The transcription cycle is divided into three key stages: initiation, elongation, and termination, each driven by specific molecular mechanisms.Initiation of TranscriptionIn bacteria, transcription begins when the RNA polymerase core enzyme associates with a sigma factor to form a holoenzyme. For example, the E. coli sigma factor called σ70 forms a holoenzyme, which recognizes the -10 (Pribnow box)...