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バシルス・サブティリス (Bacillus subtilis) の発達的に調節される遺伝子のプロモーター
Cell
|September 1, 1981
まとめ
バチルス・サブティリスの胞子化には,特定のシグマ因子 (シグマ37およびシグマ29) によって調節される発達遺伝子 (0.4kb遺伝子) が含まれる. 研究者は,この発達制御に不可欠な二重転写開始部位とプロモーター要素を特定しました.
科学分野:
- 分子生物学は分子生物学である.
- 微生物の遺伝学
- バクテリアの発達
背景:
- バチルス・サブティリスは,複雑な発育過程である胞子が形成されます.
- 特定の調節性遺伝子 (spo0 loci) は,胞子の発生を制御する.
- 胞子形成中の遺伝子転写は,発達的に調節されます.
研究 の 目的:
- 発達的に制御されたB. subtilis遺伝子 (0.4 kb遺伝子) のプロモーターのヌクレオチド配列を決定する.
- この遺伝子の転写を調節するRNAポリメラーゼシグマ因子の役割を調査する.
- プロモーターの構造と,それが発達規律に及ぼす影響を明らかにする.
主な方法:
- プロモーター領域のヌクレオチド配列. プロモーター領域のヌクレオチド配列.
- 精製されたRNAポリメラーゼとシグマ因子を用いたインビトロ転写アッセイ.
- S1核酵素マッピング,流出トランスクリプション,およびトランスクリプション開始部位を特定するためのダイヌクレオチドプライミング.
- プロモーター領域内の in vitro 消去変異の構築と分析.
主要な成果:
- 0.4kbの遺伝子プロモーターは,シグマ37とシグマ29によって認識されますが,シグマ55では認識されません.
- 約10bp離れた二重転写開始部位が,シグマ37誘導転写のために特定されました.
- 削除分析は,下流転写開始に不可欠なAT豊富な領域と上流スタートサイトを明らかにしました.
結論:
- 0.4kbの遺伝子のプロモーター構造は,その二重スタートサイトと特定のAT豊富な元素で,B. subtilisの発達的に調節された転写に不可欠です.
- シグマ37を含むRNAポリメラーゼの異なる形態は,転写開始部位に対する明確な好みを表しています.
- これらの発見は,バクテリアの発達と胞子形成の間に遺伝子発現を制御するメカニズムについての洞察を提供します.
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