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関連する概念動画

Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

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関連する実験動画

Updated: Jul 18, 2026

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
09:40

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae

Published on: September 23, 2011

T4エンドヌクレアゼVIIはホリデー構造を割る

K Mizuuchi, B Kemper, J Hays

    Cell
    |June 1, 1982
    PubMed
    まとめ

    T4内核酵素VIIはホリデイ構造を裂き,DNA複製の中間物質を分解する. DNAリゲーゼとの作用により,遺伝的に感知可能な製品が生成され,ファグT4DNA代謝におけるその役割が明らかにされる.

    科学分野:

    • 分子生物学は分子生物学である.
    • 酵素学 酵素学とは
    • DNAの複製と修復を図っています.

    背景:

    • ホリデイ構造は,DNAの再結合と修復における重要な中間物質である.
    • T4エンドヌクレアゼVIIは,バクテリオファージT4のDNA代謝に関与しています.

    研究 の 目的:

    • T4エンドヌクレアゼVIIがホリデイ構造を分解するメカニズムを解明する.
    • 遺伝的に感知可能なDNA製品を生成するT4エンドヌクレアースVIIの役割を理解する.

    主な方法:

    • 精製されたT4エンドヌクレアースVIIを用いたインビトロ分裂アッセイ.
    • エンドヌクレアースVII活性によって生成されたDNA産物の分析.

    主要な成果:

    • T4内核酵素VIIは,分岐点またはその近くでホリデイ構造の両方の鎖を特に割ります.
    • クリーバージは,2つの分岐のないDNAデュプレックスを生成し,それぞれに単一鎖の断裂がある.
    • 鎖の断裂は,各鎖の核酸配列内の同一の位置で起こります.

    結論:

    • T4内核酵素VIIは,二重複DNAの分岐点に作用し,ホリデイ構造を解除する.

    さらに関連する動画

    A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
    11:25

    A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

    Published on: March 18, 2017

    Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
    10:59

    Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

    Published on: May 24, 2017

    関連する実験動画

    Last Updated: Jul 18, 2026

    Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
    09:40

    Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae

    Published on: September 23, 2011

    A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
    11:25

    A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

    Published on: March 18, 2017

    Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
    10:59

    Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

    Published on: May 24, 2017

  • T4エンドヌクレアゼVIIとDNAリガゼの結合作用は,鎖の断裂を修復し,機能的なDNAを生成します.
  • これらの発見は,ファグT4DNA代謝における酵素の機能を説明し,特定の分岐点活性を持つ最初の酵素であると特定します.